PROJECTION OF PRO-OPIOMELANOCORTIN NEURONS FROM THE RAT ARCUATE NUCLEUS TO THE MIDBRAIN CENTRAL GRAY AS DEMONSTRATED BY DOUBLE STAINING WITH RETROGRADE LABELING AND IMMUNOHISTOCHEMISTRY
PROJECTION OF PRO-OPIOMELANOCORTIN NEURONS FROM THE RAT ARCUATE NUCLEUS TO THE MIDBRAIN CENTRAL GRAY AS DEMONSTRATED BY DOUBLE STAINING WITH RETROGRADE LABELING AND IMMUNOHISTOCHEMISTRY
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DOI：
10.1679/aohc.51.175
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发表时间：
1988-05-01
影响因子：
--
通讯作者：
TANIGUCHI, Y
中科院分区：
文献类型：
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作者：
YOSHIDA, M;TANIGUCHI, Y
将辣根过氧化物酶-麦胚凝集素偶联物（HRP-WGA偶联物）注入3只成年大鼠的中脑中央灰质（MCG）。前脑部分首先用二氨基联苯胺和过氧化氢处理，以检测逆行转运的结合物。然后将它们化学染色以检测阿黑皮素原（POMC）衍生的肽（ACTH，β-内啡肽和α- MSH）。三种POMC衍生肽的共存通过用对每种肽特异性的抗血清染色的三个连续切片的免疫组织化学来证实。弓状核内及周围部分神经元胞体对POMC衍生肽免疫组化染色呈阳性，并同时被HRP-WGA标记，表明它们投射到MCG。它们主要集中在弓状核的头端五分之三。弓状核部分POMC神经元投射到中脑中央灰质的发现值得关注，因为中央灰质参与阿片肽诱导的镇痛作用。
Conjugate of horseradish peroxidase and wheat germ agglutinin (HRP-WGA conjugate) was injected into the midbrain central gray (MCG) of three adult rats. Frontal sections of the diencephalon were first treated with diaminobenzidine and hydrogen peroxide to detect the retrogradely transported conjugate. They were then stained immunohistochemically to detect pro-opiomelanocortin (POMC)-derived peptides (ACTH, .beta.-endorphin and .alpha.-MSH). The coexistence of the three POMC-derived peptides was confirmed by the immunohistochemistry of three consecutive sections stained with antiserum specific to each peptide. Some of the neuronal perikarya distributed in and around the arcuate nucleus were positive to the immunohistochemical stain for POMC-derived peptides, and, concomitantly, were labeled with HRP-WGA conjugate, which indicated that they projected to the MCG. They were mostly concentrated in the rostral three-fifths of the arcuate nucleus. The finding that some of the POMC neurons in the arcuate nucleus project to the midbrain central gray deserves interest, because the central gray is involved in analgesia induced by opioid peptides.