Antisense transcription in the mammalian transcriptome
Antisense transcription in the mammalian transcriptome
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DOI：
10.1126/science.1112009
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发表时间：
2005-09-02
期刊：
影响因子：
56.9
通讯作者：
Wahlestedt, C
中科院分区：
文献类型：
--
作者：
Katayama, S;Tomaru, Y;Wahlestedt, C
反义转录（从相反链转录到蛋白质编码链或正义链）已被认为在基因调控中起作用，涉及相应正义转录物的降解（RNA干扰），以及在染色质水平上的基因沉默。全局转录组分析提供的证据表明，基因组的大部分可以从两条链产生转录本，并且反义转录本通常将复杂基因座中的相邻"基因"连接成连接的转录单位链。表达谱分析揭示了有义/反义对的频繁一致调节。我们目前的实验证据表明，干扰的反义RNA可以改变正义信使RNA的表达，这表明反义转录有助于控制哺乳动物的转录输出。
Antisense transcription (transcription from the opposite strand to a protein-coding or sense strand) has been ascribed roles in gene regulation involving degradation of the corresponding sense transcripts (RNA interference), as well as gene silencing at the chromatin level. Global transcriptome analysis provides evidence that a large proportion of the genome can produce transcripts from both strands, and that antisense transcripts commonly link neighboring "genes" in complex loci into chains of linked transcriptional units. Expression profiling reveals frequent concordant regulation of sense/antisense pairs. We present experimental evidence that perturbation of an antisense RNA can alter the expression of sense messenger RNAs, suggesting that antisense transcription contributes to control of transcriptional outputs in mammals.