Soil pH is a Key Determinant of Soil Fungal Community Composition in the Ny-Ålesund Region, Svalbard (High Arctic).
Soil pH is a Key Determinant of Soil Fungal Community Composition in the Ny-Ålesund Region, Svalbard (High Arctic).
复制标题
DOI：
10.3389/fmicb.2016.00227
复制
发表时间：
2016
影响因子：
5.2
通讯作者：
Yu LY
中科院分区：
文献类型：
--
作者：
Zhang T;Wang NF;Liu HY;Zhang YQ;Yu LY
本研究评估了真菌群落组成及其与Ny-斯瓦尔巴特群岛，高北极地区表层土壤性质的关系。采用454焦磷酸测序技术，对13个土壤样品的rDNA内转录间隔区（ITS）序列进行了分析。分析了土壤pH、有机碳（C）、有机氮（N）、铵态氮（NH 4 +-N）、硅酸盐硅（SiO 4 2--Si）、亚硝酸盐氮（NO2--N）、磷酸盐磷（PO 4 3--P）和硝酸盐氮（NO3--N）等8个性质。在这些OTU中，343个属于子囊菌门（Ascomycota），100个属于担子菌门（Basidiomycota），31个属于壶菌门（Chytridiomycota），22个属于球囊菌门（Glomeromycota），11个属于接合菌门（Zygomycota），10个属于Rozellomycota，而24个属于未知真菌。优势目为Helotiales、Verrucariales、Agaricales、Lecanorales、Chaetoestriales、Lecideales和Capnodiales。常见的属（> 8个土壤样品）为Tetracladium、Mortierella、Fusarium、Cortinarius和Atla。基于距离的冗余分析（db-rda）和相似性分析（ANOSIM）显示，土壤pH值（p = 0.001）是最显着的因素，在确定土壤真菌群落组成。发现疣孢目在pH 8-9的土壤中占主导地位，而囊壳目在pH 7-8的土壤中占主导地位，而Coniochaetales在pH 6-7的土壤中占主导地位。研究结果表明，北极高纬度地区土壤真菌群落多样性的存在和分布，为研究北极土壤真菌群落对气候变化的生态响应提供了可靠的数据。
This study assessed the fungal community composition and its relationships with properties of surface soils in the Ny-Ålesund Region (Svalbard, High Arctic). A total of thirteen soil samples were collected and soil fungal community was analyzed by 454 pyrosequencing with fungi-specific primers targeting the rDNA internal transcribed spacer (ITS) region. The following eight soil properties were analyzed: pH, organic carbon (C), organic nitrogen (N), ammonium nitrogen (NH4+-N), silicate silicon (SiO42--Si), nitrite nitrogen (NO2--N), phosphate phosphorus (PO43--P), and nitrate nitrogen (NO3--N). A total of 57,952 reads belonging to 541 operational taxonomic units (OTUs) were found. of these OTUs, 343 belonged to Ascomycota, 100 to Basidiomycota, 31 to Chytridiomycota, 22 to Glomeromycota, 11 to Zygomycota, 10 to Rozellomycota, whereas 24 belonged to unknown fungi. The dominant orders were Helotiales, Verrucariales, Agaricales, Lecanorales, Chaetothyriales, Lecideales, and Capnodiales. The common genera (>eight soil samples) were Tetracladium, Mortierella, Fusarium, Cortinarius, and Atla. Distance-based redundancy analysis (db-rda) and analysis of similarities (ANOSIM) revealed that soil pH (p = 0.001) was the most significant factor in determining the soil fungal community composition. Members of Verrucariales were found to predominate in soils of pH 8–9, whereas Sordariales predominated in soils of pH 7–8 and Coniochaetales predominated in soils of pH 6–7. The results suggest the presence and distribution of diverse soil fungal communities in the High Arctic, which can provide reliable data for studying the ecological responses of soil fungal communities to climate changes in the Arctic.