Papilloma formation in human foreskin xenografts after inoculation of human papillomavirus type 16 DNA.
Papilloma formation in human foreskin xenografts after inoculation of human papillomavirus type 16 DNA.
复制标题
接种人乳头瘤病毒 16 型 DNA 后人包皮异种移植物中乳头状瘤的形成。
DOI：
10.1128/jvi.69.4.2716-2721.1995
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Longley,BJ
中科院分区：
文献类型：
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作者：
Brandsma,JL;Brownstein,DG;Xiao,W;Longley,BJ
将人乳头瘤病毒（HPV） 16型DNA接种于移植到重度联合免疫缺陷（scid）小鼠皮肤上的人包皮，建立了人乳头瘤病毒感染高危小鼠模型。移植皮肤含有人表皮和真皮层，与正常人皮肤一样，在分化角质细胞中表达天合蛋白。将HPV 16型DNA与金颗粒结合，直接注入人表皮细胞，诱导外生性乳头状瘤，表现为乳头瘤病毒感染的组织学特征，包括嗜核细胞增多和乳头瘤病毒衣壳抗原的表达。该模型将有助于确定病毒基因在体内的功能，并有助于制定预防和治疗hpv相关疾病的策略。它也可能对开发其他人类皮肤病的动物模型有价值。
A mouse model of high-risk human papillomavirus infection was developed in which human papillomavirus (HPV) type 16 DNA was inoculated into human foreskin grafted to the skin of severe combined immunodeficient (scid) mice. Grafted skin contained human epidermis and dermis and, like normal human skin, expressed involucrin in differentiating keratinocytes. HPV type 16 DNA, attached to gold particles, was delivered directly into human epidermal cells and induced exophytic papilloma with histologic features of papillomavirus infection, including koilocytosis and expression of papillomavirus capsid antigen. This model should be useful for determining in vivo the functions of viral genes and for developing strategies to prevent and treat HPV-associated disease. It may also be of value in developing animal models of other human skin diseases.