Development of a passive micromixer based on repeated fluid twisting and flattening, and its application to DNA purification
Development of a passive micromixer based on repeated fluid twisting and flattening, and its application to DNA purification
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DOI：
10.1007/s00216-005-0073-y
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发表时间：
2005-11-01
影响因子：
4.3
通讯作者：
Seki, M
中科院分区：
文献类型：
--
作者：
Lee, NY;Yamada, M;Seki, M
我们开发了一种基于新混合原理、流体扭曲和扁平化的三维被动微混合器。该微混合器由重复的两个微通道段构成，即“主通道”和“扁平通道”，这两个微通道段的尺寸差异很大并且垂直排列。在这些段的交叉处，微混合器内的流体发生扭曲，然后在扁平的通道中，扩散长度大大缩短，从而实现高混合效率。制造了几种类型的微混合器，并评估了微通道几何形状对混合性能的影响。我们还将这种微混合器与小型化DNA纯化装置集成，其中缓冲溶液的浓度可以快速改变，以进行基于固相提取的DNA纯化。
We have developed a three-dimensional passive micromixer based on new mixing principles, fluid twisting and flattening. This micromixer is constructed by repeating two microchannel segments, a "main channel" and a "flattened channel", which are very different in size and are arranged perpendicularly. At the intersection of these segments the fluid inside the micromixer is twisted and then, in the flattened channel, the diffusion length is greatly reduced, achieving high mixing efficiency. Several types of micromixer were fabricated and the effect of microchannel geometry on mixing performance was evaluated. We also integrated this micromixer with a miniaturized DNA purification device, in which the concentration of the buffer solution could be rapidly changed, to perform DNA purification based on solid-phase extraction.