Stress-induced degranulation accompanied by vesicle formation in the adrenal chromaffin cells of the mouse.
Stress-induced degranulation accompanied by vesicle formation in the adrenal chromaffin cells of the mouse.
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应激诱导的脱颗粒伴随小鼠肾上腺嗜铬细胞中囊泡的形成。
DOI：
10.1679/aohc1950.42.375
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发表时间：
1979
期刊：
影响因子：
--
通讯作者：
Y. Serizawa
中科院分区：
文献类型：
--
作者：
S. Kobayashi;Y. Serizawa
将小鼠四肢固定在板子上，后足浸泡在20℃的水浴中，通过光镜和电镜观察肾上腺染色质细胞的精细结构。应激8 ~ 22 h后，光镜观察NA细胞中阿根廷蛋白颗粒数量明显减少。电镜观察，A、NA和SGC细胞颗粒数明显减少。这种脱颗粒过程伴随着大量小的细胞质囊泡的出现，这些囊泡通常是空的，但有时含有电子致密物质。含有颗粒的质膜内陷是常见的，并被解释为代表胞外炎的轮廓。分泌颗粒膜和相关物质可能以细胞质囊泡的形式被恢复的观点得到了支持。小鼠暴露于约束和水浸应激被证明是一种简单的方法，产生急性和剧烈的肾上腺染色质细胞脱颗粒。
Stress was induced in mice by restraining on a board by pinning their limbs followed by immersion of the hind feet in a water bath of 20 degrees C. Fine structure of adrenal chromaffin cells was studied by light and electron microscopy. After 8 to 22 hrs' stress, remarkable decrease in the number of argentaffin granules of the NA cells was demonstrated by light microscopy. By electron microscopy, the decline in the granule number was marked in A, NA and SGC cells. This degranulation was accompanied by theoccurrence of numerous smally cytoplasmic vesicles which often appeared empty but sometimes contained an electron-dense material. Granule-containing invaginations of plasma membrane were frequently seen and were interpreted to represent the exocytotis profiles. The idea that the secretory granule membrane and associated substances might be recovered in the form of cytoplasmic vesicles was thus supported. Exposure of the mouse to restraint plus water immersion stress proved to be a simple method for production of acute and drastic degranulation of the adrenal chromaffin cells.