Shining light on the stability of metal thiosemicarbazonate complexes in living cells by FLIM
Shining light on the stability of metal thiosemicarbazonate complexes in living cells by FLIM
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DOI：
10.1039/c2sc21489j
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发表时间：
2013-01-01
期刊：
影响因子：
8.4
通讯作者：
Dilworth, Jonathan R.
中科院分区：
文献类型：
--
作者：
Waghorn, Philip A.;Jones, Michael W.;Dilworth, Jonathan R.
Cu（II）、Ni（II）和Zn（II）的配合物已经用双（氨基硫脲）配体制备，所述配体带有具有（ATSMBodipy）和不具有（GTSBodipy）主链甲基的悬垂Bodipy荧光团。生物物理分析使用稳态共聚焦激光扫描荧光图像和激发态荧光寿命成像显微镜（FLIM）的数据从活细胞有氧摄取条件下获得的唯一允许的空间分布和解离程度的复合物进行评估。CuATSMBodipy复合物在20分钟和1小时后几乎是完整的，而相应的CuGTSBodipy复合物完全解离。络合物ZnATSMBodipy和NiATSMBodipy显示出几乎没有解离的证据。这些数据的相关性的生物医学应用的双（氨基硫脲）配合物的成像应用和影响，如阿尔茨海默氏症和PET成像的缺氧的疾病的治疗进行了讨论。
Complexes of Cu(II), Ni(II) and Zn(II) have been prepared with bis(thiosemicarbazonate) ligands bearing a pendant Bodipy fluorophore both with (ATSMBodipy) and without (GTSBodipy) backbone methyl groups. Biophysical analysis using steady state confocal laser scanning fluorescence images and excited state fluorescence lifetime imaging microscopy (FLIM) data obtained from live cells under aerobic uptake conditions has uniquely allowed both the spatial distribution and degree of dissociation of the complexes to be assessed. The CuATSMBodipy complex is virtually intact after 20 min and 1 h whereas the corresponding CuGTSBodipy complex is totally dissociated. The complexes ZnATSMBodipy and NiATSMBodipy show little evidence for dissociation. The relevance of this data to the biomedical applications of bis(thiosemicarbazonate) complexes for imaging applications and implications for the treatment of disease such as Alzheimer's and the PET imaging of hypoxia are discussed.