Magnetic nonequivalence of the two fatty acid chains in phospholipids of small unilamellar vesicles and mixed micelles.
Magnetic nonequivalence of the two fatty acid chains in phospholipids of small unilamellar vesicles and mixed micelles.
复制标题
小单层囊泡和混合胶束磷脂中两条脂肪酸链的磁性不等价。
DOI：
10.1021/bi00521a025
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发表时间：
1981
期刊：
影响因子：
2.9
通讯作者：
Dennis,EA
中科院分区：
文献类型：
--
作者：
DeBony,J;Dennis,EA
Jacqueline De Bony和Edward A. Dennis*摘要：磷脂中两个酰基链的磁不等效性已经在几种胶束中得到了证明[Roberts， M. F., Bothner-By， A. A., & Dennis， E. A.(1978) Biochemistry, 17,935]。两个a-亚甲基的化学位移已经确定，两条链之间约0.1 ppm的差异被解释为两条脂肪酸链相对于界面位置的差异。这个结论现在已经扩展到磷脂系统更接近生物膜的形式，通过磷脂分散体的超声制备的小单层囊泡。比较了几种磷脂囊泡，包括磷脂酰丝氨酸、磷脂酰甘油、磷脂酰乙醇胺、磷脂酸和磷脂酰胆碱。通过适当的分辨率增强技术，可以克服光谱中的宽谱线，在大多数磷脂囊泡中观察到sn-1和sn-2 a-亚甲基质子。sn-2链上的两个a-亚甲基质子是不相等的，并且
Jacqueline De Bony and Edward A. Dennis* abstract: Magnetic nonequivalence of the two acyl chains in phospholipids has been shownby'H NMR in several kinds of micelles [Roberts, M. F., Bothner-By, A. A., & Dennis, E. A.(1978) Biochemistry, 17,935]. The chemical shifts of the two a-methylene groups have been assigned, and a difference of about 0.1 ppm between the two chains has been interpreted as a difference in the position of the two fatty acid chains relative to the interface. This conclusion has now been ex-tended to phospholipid systems closer to biological membranes in the form of small unilamellar vesicles prepared by sonication of phospholipid dispersions. Several kinds of phospholipid vesicles are compared, including phosphatidylserine, phos-phatidylglycerol, phosphatidylethanolamine, phosphatidic acid, and phosphatidylcholine. By using suitable resolution en-hancement techniques, it was possible to overcome the broad lines in the spectra and observe the sn-1 and sn-2 a-methylene protons in most of the phospholipid vesicles. The two a-methylene protons on the sn-2 chain are nonequivalent, and