Lipoprotein lipase mRNA expression in abdominal adipose tissue is little modified by age and nutritional state in broiler chickens
Lipoprotein lipase mRNA expression in abdominal adipose tissue is little modified by age and nutritional state in broiler chickens
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DOI：
10.1093/ps/81.6.846
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发表时间：
2002-06-01
期刊：
影响因子：
4.4
通讯作者：
Akiba, Y
中科院分区：
文献类型：
--
作者：
Sato, K;Akiba, Y
脂蛋白脂酶（LPL）催化血浆脂蛋白水解是肉鸡外周组织脂质转运的限速步骤。本研究的目的是探讨脂肪组织中LPL mRNA的表达是否受到年龄或营养处理的影响，以期减少肉鸡的脂肪积累。结果表明：4、6、8周龄鸡腹部脂肪组织中LPL mRNA的表达量差异不显著，而2周龄鸡腹部脂肪组织中LPL mRNA的表达量较低。在营养调节中，腹部脂肪组织中的LPL mRNA水平没有被48小时的饲料剥夺或随后的再喂养48小时所改变。此外，LPL mRNA的表达没有显着改变，在鸡喂养7天的饮食含有8%的橄榄油（富含三油酸甘油酯），红花油（富含三亚油酸甘油酯），或亚麻籽油（富含三亚麻酸甘油酯）。另一方面，脂肪LPL mRNA的表达在鸡强制喂食12小时与trilinolenin（18：3）乳液后48小时的饲料剥夺显着降低时，在鸡强制喂食与triolein（18：1）或trilinolein（18：2）乳液。鸡腹部脂肪组织中LPL免疫反应蛋白水平的变化所带来的老化和营养的操作是类似的观察到的mRNA的表达。这些结果表明，LPL mRNA的表达在生长鸡是响应老化和营养操纵比哺乳动物，从而表明肉鸡LPL生理反应的特异性。
Lipoprotein lipase (LPL)-catalyzed hydrolysis of plasma lipoproteins is a rate-limiting step in the transport of lipids into the peripheral tissues of broiler chickens. The aim of the present study was to investigate whether LPL mRNA expression in adipose tissue is affected by age or nutritional treatments, with a view to reducing fat accumulation in broiler chickens. The study found that chicken LPL mRNA expression in abdominal adipose tissue did not differ significantly between chickens aged 4, 6, and 8 wk, but there was less expression of LPL mRNA in 2-wk-old chickens. In nutritional modulation, LPL mRNA levels in abdominal adipose tissues were not modified by 48-h feed deprivation or by subsequent refeeding for 48 h. In addition, expression of LPL mRNA was not significantly altered in chickens fed for 7 d on diets containing 8% olive oil (triolein rich), safflower oil (trilinolein rich), or linseed oil (trilinolenin rich). On the other hand, adipose LPL mRNA expression in chickens force-fed for 12 h with a trilinolenin (18:3) emulsion after 48-h feed deprivation was significantly decreased when compared to that in chickens force-fed with a triolein (18:1) or trilinolein (18:2) emulsion. Changes to LPL immunoreactive protein levels in chicken abdominal adipose tissues brought about by aging and nutritional manipulations were similar to those observed in relation to mRNA expression. These findings suggest that LPL mRNA expression in growing chickens is less responsive to aging and nutritional manipulation than in mammals, thereby indicating specificity of physiological response on broiler chicken LPL.