Proteomics technique opens new frontiers in mobilome research.
Proteomics technique opens new frontiers in mobilome research.
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DOI：
10.1080/2159256x.2017.1362494
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Maringer K
中科院分区：
文献类型：
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作者：
Davidson AD;Matthews DA;Maringer K
大多数真核生物的基因组的大部分由高度重复的移动的遗传元件组成。这些元素的总和被称为“移动体”，在真核生物中主要由转座子组成。转座因子通过介导基因重排以及通过转座子蛋白的“驯化”来实现细胞功能，从而对疾病、进化和正常生理机能做出贡献。虽然对活动组基因组和转录组的组学研究很常见，但技术挑战阻碍了转座子的高通量全球蛋白质组学分析。在最近的一篇论文中，我们使用一种名为“转录组学通知的蛋白质组学”（PIT）的技术克服了这些技术障碍，从而发表了第一个无偏见的全球mobilome衍生的蛋白质组（使用来自埃及伊蚊的细胞系）。在这篇评论中，我们更详细地描述了我们的方法，并总结了我们的主要发现。我们还使用新的基因组测序数据表明，在许多情况下，表达给定蛋白质的特定基因组元件可以使用PIT进行鉴定。因此，这种蛋白质组学技术代表了一种重要的技术进步，它将为研究转座子和其他重复和序列多样性遗传元件（如内源性逆转录病毒）衍生的蛋白质在健康和疾病中的作用开辟新的途径。
A large proportion of the genome of most eukaryotic organisms consists of highly repetitive mobile genetic elements. The sum of these elements is called the “mobilome,” which in eukaryotes is made up mostly of transposons. Transposable elements contribute to disease, evolution, and normal physiology by mediating genetic rearrangement, and through the “domestication” of transposon proteins for cellular functions. Although ‘omics studies of mobilome genomes and transcriptomes are common, technical challenges have hampered high-throughput global proteomics analyses of transposons. In a recent paper, we overcame these technical hurdles using a technique called “proteomics informed by transcriptomics” (PIT), and thus published the first unbiased global mobilome-derived proteome for any organism (using cell lines derived from the mosquito Aedes aegypti). In this commentary, we describe our methods in more detail, and summarise our major findings. We also use new genome sequencing data to show that, in many cases, the specific genomic element expressing a given protein can be identified using PIT. This proteomic technique therefore represents an important technological advance that will open new avenues of research into the role that proteins derived from transposons and other repetitive and sequence diverse genetic elements, such as endogenous retroviruses, play in health and disease.