Trends in the gentamicin and arbekacin susceptibility of methicillin-resistant Staphylococcus aureus and the genes encoding aminoglycoside-modifying enzymes
Trends in the gentamicin and arbekacin susceptibility of methicillin-resistant Staphylococcus aureus and the genes encoding aminoglycoside-modifying enzymes
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耐甲氧西林金黄色葡萄球菌对庆大霉素和阿贝卡星的敏感性变化及氨基糖苷类修饰酶基因的变化
DOI：
10.1007/s10156-006-0497-0
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发表时间：
2007
影响因子：
2.2
通讯作者：
K. Sunakawa
中科院分区：
文献类型：
--
作者：
Kazunari Barada;H. Hanaki;S. Ikeda;Y. Yamaguchi;H. Akama;T. Nakae;T. Inamatsu;K. Sunakawa
一般认为耐甲氧西林金黄色葡萄球菌（MRSA）对氨基糖苷类抗生素也有耐药性。我们调查了庆大霉素和阿贝卡星药敏性的趋势和编码氨基糖苷类修饰酶（艾姆斯）的基因的流行率，共218株MRSA分离自1978年至2002年在一家医院的血液标本。对于1978年至1984年和1985年至1989年获得的分离株，50%菌株被抑制时庆大霉素的最低抑菌浓度（MIC 50）分别为≥128和32 µg/ml，对于1990年至2002年获得的分离株，MIC 50为0.5 µg/ml。庆大霉素的MIC 90始终≥128 µg/ml。对AME发生的研究表明，庆大霉素的MIC 50与编码氨基糖苷乙酰/磷酸转移酶的aac（6′）/aph（2″）的存在高度相关。对于1978-1984年分离的菌株，阿贝卡星的MIC 50为2 µg/ml，对于1985 - 2002年分离的菌株，其MIC 50 ≤0.5 µg/ml。对于1978-1989年分离的菌株，阿贝卡星的MIC 90为8 µg/ml，对于1990-2002年分离的菌株，为1 - 2 µg/ml。虽然已经确定AAC（6′）/APH（2″）修饰阿贝卡星，但阿贝卡星耐药的趋势不一定与该酶的存在一致。然而，1978年至2002年分离的菌株中aac（6′）/aph（2″）和aph（3′）-III的流行率与阿贝卡星的MIC 90值相关。因此，除AAC（6′）/APH（2″）外，APH（3′）-III很可能在某种程度上参与了S.金黄色。我们的研究结果表明，庆大霉素和阿贝卡星耐药MRSA在过去25年中一直在下降，这一发现很可能是由于编码艾姆斯的基因的流行率下降。
It is generally accepted that methicillin-resistant Staphylococcus aureus (MRSA) is also resistant to aminoglycoside antibiotics. We investigated trends of gentamicin and arbekacin susceptibilities and the prevalence of the genes encoding aminoglycoside-modifying enzymes (AMEs) for a total of 218 strains of MRSA isolated from blood specimens obtained from 1978 through 2002 in one hospital. The minimum inhibitory concentrations of gentamicin at which 50% of the strains were inhibited (MIC50) were ≥128 and 32 µg/ml for isolates obtained from 1978 to 1984 and from 1985 to 1989, respectively, and 0.5 µg/ml for isolates obtained from 1990 to 2002. The MIC90 of gentamicin was consistently ≥128 µg/ml. Investigation of the occurrence of AME revealed that the MIC50 of gentamicin was highly correlated with the presence of aac(6′)/aph(2″) encoding aminoglycoside acetyl/phosphotransferase. The MIC50 of arbekacin was 2 µg/ml for strains isolated in 1978–1984 and ≤0.5 µg/ml for strains isolated from 1985 to 2002. The MIC90 of arbekacin was 8 µg/ml for the strains isolated in 1978–1989 and 1 to 2 µg/ml for strains isolated in 1990–2002. Though it has been established that AAC(6′)/APH(2″) modifies arbekacin, the trend of arbekacin resistance was not necessarily consistent with the presence of this enzyme. However, the prevalence of both aac(6′)/aph(2″) and aph(3′)-III in the strains isolated from 1978 through 2002 was correlated with the MIC90 values of arbekacin. Thus, it is most likely that APH(3′)-III, in addition to AAC(6′)/APH(2″), is somehow involved in arbekacin resistance in S. aureus. Our results imply that gentamicin- and arbekacin-resistant MRSAs have consistently decreased for the past 25 years and that this finding is, most likely, attributable to the declining prevalence of genes encoding for AMEs.
日本医院中阿贝卡星耐药 MRSA 菌株的趋势
DOI：
--
发表时间：
2006
期刊：
J. Antibiot. 59
影响因子：
--
作者：
Tsuchizaki;N.;Ishino;K.;Saito;F. Ishikawa;J.;Nakajima;M.;Hotta;K.
通讯作者：
K.
用于快速检测 MRSA 和肠球菌中抗生素抗性基因的菌落 PCR。
DOI：
--
发表时间：
2000
期刊：
Japanese J. Antibiotics 53(6)
影响因子：
--
作者：
Tsuchizaki N;Ishikawa J;Hotta K
通讯作者：
Hotta K