A microsatellite linkage map of Barramundi, Lates calcarifer
A microsatellite linkage map of Barramundi, Lates calcarifer
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DOI：
10.1534/genetics.106.059972
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发表时间：
2007-02-01
期刊：
影响因子：
3.3
通讯作者：
Yue, Gen Hua
中科院分区：
文献类型：
--
作者：
Wang, Chun Ming;Zhu, Ze Yuan;Yue, Gen Hua
尖吻鲈（Lates calcarifer）是一种重要的海水养殖食用鱼类。其紧凑的基因组（约700Mb）是食用鱼类中最小的基因组之一。我们利用一个包含三个亲本（两雄一雌）和93个子代的作图群体构建了尖吻鲈的第一代遗传连锁图谱。共有240个微卫星标记被定位到24个连锁群中。在这些标记中，10个位于表达序列标签（ESTs）和已知基因中。雌性和雄性图谱的总长度分别为873.8和414.5厘摩（cM），平均标记间距分别为6.20和4.70厘摩。将240个尖吻鲈微卫星的侧翼序列与黑青斑河豚（Tetraodon nigrovidiris）的全基因组组装序列进行比较，发现55个同源序列位于黑青斑河豚21条染色体中的19条上。该图谱不仅能够用于数量性状基因座的定位，还为理解鱼类基因组的进化提供了新的资源。
Barramundi (Lates calcarifer) is an important farmed marine food fish species. Its compact genome (similar to 700 Mb) is among the smallest genomes of food fish species. We established a first-generation genetic linkage map of Barramundi with a mapping panel containing three parents (two males and one female) and 93 progeny. A total of 240 microsatellite markets were mapped into 24 linkage groups. Among these markers, 10 were located in ESTs and known genes. The total lengths of the female and male maps were 873.8 and 414.5 cM with an average marker spacing of 6.20 and 4.70 cM, respectively. Comparing the flanking sequences of the 240 Barramundi microsatellites with the assembled whole-genome sequences of Tetraodon, nigrovidiris revealed 55 homologous sequences located in 19 of the 21 chromosomes of T. nigrovidiris. The map ill not only enable the mapping of quantitative trait loci, but also provide new resources for understanding the evolution of fish genomes.