A sensitive assay of transthyretin (prealbumin) in human cerebrospinal fluid in nanogram amounts by ELISA.
A sensitive assay of transthyretin (prealbumin) in human cerebrospinal fluid in nanogram amounts by ELISA.
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DOI：
10.1016/0009-8981(91)90344-c
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发表时间：
1991-02
期刊：
影响因子：
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通讯作者：
G. Vatassery;H. Quach;W. Smith;B. Benson;J. Eckfeldt
中科院分区：
文献类型：
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作者：
G. Vatassery;H. Quach;W. Smith;B. Benson;J. Eckfeldt
本文报道了一种灵敏的测定人脑脊液(CSF)中反式甲状腺激素(前白蛋白)的酶联免疫吸附试验方法。该方法利用羊抗人反式甲状腺激素抗体(Ig G组分)捕获，用过氧化物酶偶联抗体显色。该检测方法的线性范围为每孔添加1-4 ng转甲状腺素。日内和日间变异系数分别为5.1%和6.1%。脑脊液中转甲状腺素浓度(5~20 mg/L)与相应的血清浓度(170~420 mg/L)呈显著正相关。这表明，在正常受试者的大脑和外周组织中，转甲状腺素的合成受到类似的生物控制。脑脊液中促甲状腺激素浓度与脑脊液总蛋白浓度或受试者年龄无关。
A sensitive ELISA method for determining transthyretin (prealbumin) in human cerebrospinal fluid (CSF) is described. The method utilizes goat antihuman transthyretin antibody (IgG fraction) for capture and peroxidase conjugated antibody for color development. The assay has a linear range of 1–4 ng transthyretin added per well. The within-day and between-day coefficients of variation are 5.1 and 6.1%, respectively. The concentration of transthyretin in CSF (ranging from 5 to 20 mg per L) correlated significantly with the corresponding serum concentrations (range 170–420 mg/l). This suggests that synthesis of transthyretin in the brain and peripheral tissues is under similar biological control in normal subjects. The transthyretin concentrations in CSF did not correlate with total CSF protein concentration or age of the subject.