RESTRICTED PROLIFERATION AND MIGRATION OF POSTNATALLY GENERATED NEURONS DERIVED FROM THE FOREBRAIN SUBVENTRICULAR ZONE
RESTRICTED PROLIFERATION AND MIGRATION OF POSTNATALLY GENERATED NEURONS DERIVED FROM THE FOREBRAIN SUBVENTRICULAR ZONE
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DOI：
10.1016/0896-6273(93)90281-u
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发表时间：
1993-07-01
期刊：
影响因子：
16.2
通讯作者：
LUSKIN, MB
中科院分区：
文献类型：
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作者：
LUSKIN, MB
出生后前脑的室下区主要产生胶质细胞，尽管它支持有限的神经发生。为了确定脑室下区是否具有位置特异性，我们分析了沿侧脑室前后轴的脑室下区细胞后代的表型和目的地。将含有大肠杆菌报告基因lacZ的逆转录病毒谱系示踪剂注射到新生大鼠幼鼠脑室下区的不同部位，并在此后的不同时间，用组织化学或免疫组织化学方法检测感染细胞后代中β -半乳糖苷酶的表达。脑室下区（SVZa）前部的一个离散区域产生了大量的神经元，这些神经元分化为嗅球的颗粒细胞和肾小球周围细胞——两种主要的中间神经元。因此，SVZa似乎构成了神经元祖细胞的一个特殊来源。为了到达嗅球，SVZa中产生的神经元沿着一条高度受限的路线移动了几毫米。必须有引导线索来阻止这些迁移神经元的广泛分散。
The subventricular zone of the postnatal forebrain produces mainly glia, although it supports limited neurogenesis. To determine whether the subventricular zone is positionally specified, the phenotype and destination of the progeny of subventricular zone cells along the anterior-posterior axis of the lateral ventricles were analyzed. A retroviral lineage tracer containing the E. coli reporter gene lacZ was injected into different parts of the subventricular zone of neonatal rat pups, and at various times thereafter, the expression of beta-galactosidase was detected histochemically or immunohistochemically in the descendants of infected cells. A discrete region of the anterior part of the subventricular zone (SVZa) generated an immense number of neurons that differentiated into granule cells and periglomerular cells of the olfactory bulb-the two major types of interneurons. Thus, the SVZa appears to constitute a specialized source of neuronal progenitor cells. To reach the olfactory bulb, neurons arising in the SVZa migrate several millimeters along a highly restricted route. Guidance cues must be involved to prohibit widespread dispersion of these migrating neurons.