Oriented bone formation using biomimetic fibrin hydrogels with three-dimensional patterned bone matrices.
Oriented bone formation using biomimetic fibrin hydrogels with three-dimensional patterned bone matrices.
复制标题
使用具有三维图案骨基质的仿生纤维蛋白水凝胶定向骨形成。
DOI：
10.1002/jbm.a.35212
复制
发表时间：
2015
影响因子：
4.9
通讯作者：
Imazato S.
中科院分区：
文献类型：
--
作者：
Sasaki JI;Matsumoto T;Imazato S.
皮质骨通过羟基磷灰石的内部取向发挥其生物学特性。最近，我们成功地使用纤维蛋白凝胶制造了骨基质的三维（3D）图案。我们假设这些纤维蛋白凝胶会在体内诱导皮质骨样组织，并为仿生材料提供广阔的前景。本研究的目的是评估体外成骨细胞诱导的纤维蛋白凝胶的特性，并评估具有仿生基质的纤维蛋白凝胶是否在体内形成皮质骨样组织。当含有骨髓基质细胞（BMSC）的纤维蛋白凝胶被单轴固定并培养时，BMSC 变得平行于固定方向排列。培养 50 天后，凝胶的拉伸强度从 1.5 kPa 急剧增加到 218 kPa。此外，傅里叶变换红外光谱显示纤维蛋白凝胶成分变成了天然骨的成分。对特定成分的定量分析表明，凝胶中钙和骨桥蛋白的含量随着培养时间的延长而增加。皮下植入免疫缺陷小鼠后，形成了具有层状结构的皮质骨样组织。结果表明，3D 图案化骨基质在体内诱导 3D 图案化皮质骨形成。这些含有 3D 图案骨基质的仿生材料是骨再生医学领域有前途的工具。 © 2014 Wiley periodicals, Inc. J Biomed Mater Res Part A: 103A: 622–627, 2015。
Cortical bone exerts its biological properties through internal orientations of hydroxyapatite. Recently, we succeeded in fabricating three‐dimensional (3D) patterns of bone matrices using fibrin gels. We hypothesized that these fibrin gels would induce cortical bone‐like tissuein vivoand offer promising prospects for biomimetic materials. The purposes of this study were to assess the characteristics of osteoblastic‐induced fibrin gelsin vitroand evaluate whether fibrin gels with biomimetic matrices formed cortical bone‐like tissuein vivo. When bone marrow stromal cell (BMSC)‐containing fibrin gels were uniaxially fixed and cultured, BMSCs became aligned parallel to the fixed direction. The tensile strengths of the gels increased dramatically from 1.5 to 218 kPa after 50 days in culture. Furthermore, Fourier‐transform infrared spectroscopy revealed that the fibrin gel constituents changed into those of native bone. Quantitative analyses of specific components demonstrated that the amounts of calcium and osteopontin in the gels increased with prolonged culture. After subcutaneous implantation into immunodeficient mice, cortical bone‐like tissues that possessed layered structures were formed. The results indicated that 3D patterned bone matrices induced 3D patterned cortical bone formationin vivo. These biomimetic materials containing 3D patterned bone matrices are promising tools for the field of bone regenerative medicine. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 622–627, 2015.