AN EVALUATION OF TECHNIQUES FOR MEASURING VESICULAR ARBUSCULAR MYCORRHIZAL INFECTION IN ROOTS
AN EVALUATION OF TECHNIQUES FOR MEASURING VESICULAR ARBUSCULAR MYCORRHIZAL INFECTION IN ROOTS
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DOI：
10.1111/j.1469-8137.1980.tb04556.x
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发表时间：
1980-01-01
期刊：
影响因子：
9.4
通讯作者：
MOSSE, B
中科院分区：
文献类型：
--
作者：
GIOVANNETTI, M;MOSSE, B
感染评估是许多涉及VA菌根的研究的重要组成部分。对所使用的技术范围作了总结。我们根据随机放置在培养皿或载玻片上的染色根样本的观察，计算了四种评估方法的标准误差。这些方法是基于根/网格交叉点是否存在感染，真菌占据皮层百分比的视觉估计或长度的估计，或载玻片上根片是否存在感染。可以从所提供的曲线中读出给定标准误差%感染所需的重复观察次数。讨论了不同评估方法的优点，并给出了为什么它们都可能高估真实价值的原因。
Assessment of infection is an essential part of many studies involving VA mycorrhiza. A summary is given of the range of techniques that have been used. We calculated the standard error of four methods of assessment based on observations of stained root samples either randomly arranged in a petri dish or mounted on microscope slides. The methods are based on presence or absence of infection at root/grid intersect points, on a visual estimate of percentage cortex occupied by fungus or on estimates of length, or presence or absence of infection in root pieces mounted on slides. The number of replicate observations required for a given standard error% infection can be read from the curves provided. The advantages of the different methods of assessment are discussed and reasons given why they all probably overestimate the true values.