Protein liposomes-mediated targeted acetylcholinesterase gene delivery for effective liver cancer therapy.
Protein liposomes-mediated targeted acetylcholinesterase gene delivery for effective liver cancer therapy.
复制标题
蛋白质脂质体介导的靶向乙酰胆碱酯酶基因递送用于有效的肝癌治疗
DOI：
10.1186/s12951-021-00777-9
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发表时间：
2021-01-22
影响因子：
10.2
通讯作者：
Liang X
中科院分区：
文献类型：
--
作者：
Wang K;Shang F;Chen D;Cao T;Wang X;Jiao J;He S;Liang X
研究背景如何有效地将治疗基因导入实体瘤并提高其生物利用度是当前基因治疗医学研究的主要挑战。开发高效的肿瘤靶向性非病毒基因载体在肿瘤治疗领域具有重要的应用价值。蛋白脂质结合肿瘤靶向性的功能蛋白和优良的基因传递性能，显示出潜在的靶向基因therapy. ResultsIn，我们制备转铁蛋白修饰的脂质体（Tf-PL）的乙酰胆碱酯酶（AChE）治疗基因的肝癌靶向传递。结果表明，Tf-PL/AChE脂质体的转染效率明显高于Lipo 2000，体外对肝癌细胞SMMC-7721具有良好的靶向性，体内实验表明，Tf-PL/AChE脂质体能有效靶向肝癌细胞，皮下注射转铁蛋白可明显抑制裸鼠肝癌移植瘤的生长。改良的蛋白脂质介导的基因递送策略用于靶向肝癌治疗，这对于精确的个体化癌症治疗具有很大的潜力。
BackgroundEffective methods to deliver therapeutic genes to solid tumors and improve their bioavailability are the main challenges of current medical research on gene therapy. The development of efficient non-viral gene vector with tumor-targeting has very important application value in the field of cancer therapy. Proteolipid integrated with tumor-targeting potential of functional protein and excellent gene delivery performance has shown potential for targeted gene therapy.ResultsHerein, we prepared transferrin-modified liposomes (Tf-PL) for the targeted delivery of acetylcholinesterase (AChE) therapeutic gene to liver cancer. We found that the derived Tf-PL/AChE liposomes exhibited much higher transfection efficiency than the commercial product Lipo 2000 and shown premium targeting efficacy to liver cancer SMMC-7721 cellsin vitro.In vivo, the Tf-PL/AChE could effectively target liver cancer, and significantly inhibit the growth of liver cancer xenografts grafted in nude mice by subcutaneous administration.ConclusionsThis study proposed a transferrin-modified proteolipid-mediated gene delivery strategy for targeted liver cancer treatment, which has a promising potential for precise personalized cancer therapy.
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DOI：
10.1083/jcb.201710051
发表时间：
2018-07-02
期刊：
The Journal of cell biology
影响因子：
--
作者：
MacDonald E;Brown L;Selvais A;Liu H;Waring T;Newman D;Bithell J;Grimes D;Urbé S;Clague MJ;Zech T
通讯作者：
Zech T
影响因子：
2.9
作者：
Kleven MD;Jue S;Enns CA
通讯作者：
Enns CA
影响因子：
4.9
作者：
Chen, Liyu;Simpson, Joshua D.;Thurecht, Kristofer J.
通讯作者：
Thurecht, Kristofer J.
影响因子：
8
作者：
Herrmann, Andreas;Nagao, Toshikage;Yu, Hua
通讯作者：
Yu, Hua
影响因子：
11.2
作者：
McDermott U;Ames RY;Iafrate AJ;Maheswaran S;Stubbs H;Greninger P;McCutcheon K;Milano R;Tam A;Lee DY;Lucien L;Brannigan BW;Ulkus LE;Ma XJ;Erlander MG;Haber DA;Sharma SV;Settleman J
通讯作者：
Settleman J