rK39 Enzyme-Linked Immunosorbent Assay for Diagnosis of Leishmania donovani Infection
rK39 Enzyme-Linked Immunosorbent Assay for Diagnosis of Leishmania donovani Infection
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rK39 酶联免疫吸附测定用于诊断杜氏利什曼原虫感染
DOI：
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发表时间：
1998
期刊：
影响因子：
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通讯作者：
H. Ghalib
中科院分区：
文献类型：
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作者：
E. Zijlstra;N. Daifalla;P. Kager;E. Khalil;A. Elhassan;S. Reed;H. Ghalib
摘要对苏丹地区杜氏利什曼原虫感染的rK39酶联免疫吸附试验(ELISA)和直接凝集试验(DAT)进行了比较。RK39-EL ISA对黑热病的诊断比DAT更敏感(分别为93%和80%)，两种试验在治疗后24个月仍可保持阳性。对于黑热病后皮肤利什曼病患者和亚临床感染患者，rK39-EL ISA的检测结果与∼检测结果相当，但可提前6个月发现40%的患者感染。在利什曼宁皮肤试验(LST)阳性的个体中也发生了DAT和rK39的转换，这表明在这些推测免疫的个体中存在活跃的寄生虫复制(rK39是一种无鞭毛体抗原)。与DAT相比，rK39-ELISA还检测到随机选择的LST阳性个体(六分之四)和地方性(LST阴性)对照(五分之一)中的感染。RK39酶联免疫吸附试验比DAT更敏感，可能成为流行病学研究的重要工具。
ABSTRACT The rK39 enzyme-linked immunosorbent assay (ELISA) was compared with the direct agglutination test (DAT) for Leishmania donovani infection in the Sudan. rK39 ELISA proved more sensitive than DAT in diagnosis of kala-azar (93 and 80%, respectively); both tests may remain positive up to 24 months after treatment. For patients with post-kala-azar dermal leishmaniasis and individuals with subclinical infection, rK39 ELISA performed as well as DAT but could detect infection 6 months earlier in ∼40% of patients. Conversion in DAT and rK39 ELISA also occurred in leishmanin skin test (LST)-positive individuals, suggesting active parasite replication (rK39 is an amastigote antigen) in these presumably immune individuals. In contrast to DAT, rK39 ELISA also detected infection in randomly selected LST-positive individuals (in four of six) and endemicity (LST-negative) controls (in one of five). rK39 ELISA appears more sensitive than DAT and may prove an important tool in epidemiological studies.