A Novel Method for Chronic Social Defeat Stress in Female Mice
A Novel Method for Chronic Social Defeat Stress in Female Mice
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DOI：
10.1038/npp.2017.259
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发表时间：
2018-05-01
影响因子：
7.6
通讯作者：
Gordon, Joshua A.
中科院分区：
文献类型：
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作者：
Harris, Alexander Z.;Atsak, Piray;Gordon, Joshua A.
从历史上看，临床前压力研究经常忽略女性受试者，尽管有证据表明女性的焦虑和抑郁率更高。在啮齿类动物中，许多压力易感性和恢复力的研究都集中在雄性身上，因为一种常用的范式——慢性社会失败压力——已被证明在雌性身上实施具有挑战性。我们报告了一个新版本的社会失败范式，在雌性小鼠中起作用。通过对雌性施加雄性气味来增加雌性的攻击行为，我们发现雌性小鼠经历了反复的社会失败压力，并产生了社会回避，降低了蔗糖偏好，并且相对于对照小鼠减少了在高加迷宫张开双臂的时间。此外，在这种模式下，雌性小鼠的一个子集显示出弹性，保持社会探索和蔗糖偏好的控制水平。这种方法产生的结果与在雄性小鼠中获得的结果相当，将极大地促进研究雌性应激易感性。
Historically, preclinical stress studies have often omitted , female subjects, despite evidence that women have higher rates of anxiety and depression. In rodents, many stress susceptibility and resilience studies have focused on males as one commonly used paradigm-chronic social defeat stress-has proven challenging to implement in females. We report a new version of the social defeat paradigm that works in female mice. By applying male odorants to females to increase resident male aggressive behavior, we find that female mice undergo repeated social defeat stress and develop social avoidance, decreased sucrose preference, and decreased time in the open arms of the elevated plus maze relative to control mice. Moreover, a subset of the female mice in this paradigm display resilience, maintaining control levels of social exploration and sucrose preference. This method produces comparable results to those obtained in male mice and will greatly facilitate studying female stress susceptibility.