Calcium Response and FcεRI Expression in Bone Marrow-Derived Mast Cells Co-cultured with SCG Neurites
Calcium Response and FcεRI Expression in Bone Marrow-Derived Mast Cells Co-cultured with SCG Neurites
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DOI：
10.1248/bpb.28.1963
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发表时间：
2005-10
影响因子：
2
通讯作者：
Akio Suzuki;R. Suzuki;T. Furuno;R. Teshima;M. Nakanishi
中科院分区：
文献类型：
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作者：
Akio Suzuki;R. Suzuki;T. Furuno;R. Teshima;M. Nakanishi
神经与肥大细胞之间的通讯是神经 - 免疫相互作用的典型例证。众多研究表明，对神经的刺激（或添加神经递质）能够引发肥大细胞的激活，并且肥大细胞衍生的介质能够影响神经元活动。然而，目前仍不清楚高亲和力IgE受体（FceRI）自身是否直接参与神经与肥大细胞之间的通讯。在本实验中，我们采用了一种体外共培养方法，该方法涵盖了免疫细胞（骨髓源性肥大细胞，BMMCs）与神经细胞（颈上神经节，SCG）之间的相互作用，以解决上述问题。我们发现，在共培养系统中，当BMMCs与SCG神经突相关联时，抗原（DNP7 - BSA）刺激后BMMCs内的细胞内钙离子浓度（[Ca2 +]i）升高幅度更大。但当BMMCs不与神经突相关联时，其[Ca2 +]i升高幅度较小。此外，BMMCs与SCG神经突进行3天的体外共培养显示，附着在神经突上的BMMCs质膜上FceRI表达增加。相反，位于BMMCs质膜与SCG神经突界面处的N - 钙黏蛋白分子在3天的共培养过程中并未增加。所有这些结果表明，BMMCs与SCG神经突共培养3天不仅促进了BMMCs中的钙反应，还促进了FceRI的表达。
Communication between nerves and mast cells is a prototypic demonstration of neuro-immune interaction. Numerous studies have shown that the stimulation of nerves (or addition of neurotransmitters) can evoke activation of mast cells, and that mast cell-derived mediators can influence neuronal activity. However, it is still unknown whether high affinity IgE receptors (FceRI) themselves are involved directly in the communication between nerves and mast cells. In the present experiments, we used an in vitro co-culture approach comprising interaction between immune (bone marrow-derived mast cells, BMMCs) and nerve cells (superior cervical ganglia, SCG) to solve the above problem. We found that the intracellular calcium ion concentration ([Ca2+]i) increased much more in BMMCs after antigen (DNP7-BSA) stimulation when they were associated with SCG neurites in the co-culture system. But the [Ca2+]i in BMMCs was less increased when they were not associated with the neurites. Further, the in vitro co-culture approach of BMMCs with SCG neurites for 3 d showed the increases of FceRI expression occurred on the plasma membranes of BMMCs which were attached to the neurites. On the contrary, N-cadherin molecules which localized on the interface between on the plasma membrane of BMMCs and SCG neurites did not increase with the co-culture for 3 d. All of these results indicated that co-culturing BMMCs with SCG neurites for 3 d promoted not only the calcium response but also the FceRI expression in BMMCs.