Low long non-coding RNA HOTAIR expression is associated with down-regulation of Nrf2 in the spermatozoa of patients with asthenozoospermia or oligoasthenozoospermia.
Low long non-coding RNA HOTAIR expression is associated with down-regulation of Nrf2 in the spermatozoa of patients with asthenozoospermia or oligoasthenozoospermia.
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发表时间：
2015-11
影响因子：
1.4
通讯作者：
Li-Xing Zhang;Zhi‐neng Liu;Xiaokang Li;Ping Zhang;Jia Wang;Dandan Zhu;Xinping Chen;L. Ye
中科院分区：
文献类型：
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作者：
Li-Xing Zhang;Zhi‐neng Liu;Xiaokang Li;Ping Zhang;Jia Wang;Dandan Zhu;Xinping Chen;L. Ye
HOTAIR是一种长链非编码RNA，调节肿瘤细胞的发生和发展以及正常干细胞的功能。然而，HOTAIR在弱精子症和少弱精子症患者精子中的作用及其分子机制尚不清楚。在此，45名健康对照、45名弱精子症患者和45名少弱精子症患者入选。最初，通过分析HOTAIR表达，我们观察到患者中HOTAIR表达水平降低。随后，我们发现患者中HOTAIR表达与Nrf2表达之间呈正相关。HOTAIR的低表达也被观察到与特定的精子功能参数相关，包括运动力和活力。在患者射出的精子中，观察到Nrf2基因启动子的组蛋白H4乙酰化水平较低。最后，我们发现HOTAIR表达的下调降低了Nrf2启动子中组蛋白H4的乙酰化和Nrf2的表达。因此，本研究表明HOTAIR在弱精子症和少弱精子症患者精子中的表达较低，这导致Nrf2表达下调。我们的数据表明HOTAIR表达的降低导致了精子功能中ROS相关的缺陷。
HOTAIR, a long noncoding RNA, regulates development and progression of tumor cells and function of normal stem cells. However, the role and the molecular mechanism of HOTAIR in the spermatozoa of patients with asthenozoospermia and oligoasthenozoospermia are still unclear. Herein, 45 healthy control, 45 asthenozoospermic patients and 45 oligoasthenozoospermic patients were enrolled. Initially, through analyzing HOTAIR expression, we observed a decreased level of HOTAIR expression in patients. Subsequently, we found that there was a positive correlation between HOTAIR expression and Nrf2 expression in patients. The low expression of HOTAIR was also observed to be associated with specific sperm function parameters, including motility and vitality. In the ejaculated spermatozoa from patients, low level of histone H4 acetylation of the Nrf2 gene promoter was observed. Finally, we found that downregulation of HOTAIR expression reduced histone H4 acetylation in Nrf2 promoter and Nrf2 expression. Therefore, this study demonstrated that HOTAIR expression was low in the spermatozoa of patients with asthenozoospermia and oligoasthenozoospermia, which resulted in down-regulation of Nrf2 expression. Our data suggested the decrease of HOTAIR expression led to ROS related defects in sperm function.