Production of tumor necrosis factor-alpha by alveolar macrophages of lung cancer patients.
Production of tumor necrosis factor-alpha by alveolar macrophages of lung cancer patients.
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DOI：
10.1111/j.1349-7006.1990.tb02582.x
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发表时间：
1990-04
期刊：
影响因子：
--
通讯作者：
Ogura T
中科院分区：
文献类型：
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作者：
Okubo A;Sone S;Singh SM;Ogura T
我们比较了健康供体和肺癌患者肺泡巨噬细胞（AM）与脂多糖（LPS）活化后的血液单核细胞产生肿瘤坏死因子（TNF）的能力。通过对放线菌素D处理的L929细胞的细胞毒性测定TNF的活性，并采用针对TNF α的多克隆和单克隆抗体夹心酶联免疫吸附法（ELISA）定量测定TNF。来自健康供体的未刺激AM自发释放可变数量的TNF，而血液单核细胞则没有。当用LPS处理24小时时，AM和单核细胞产生TNF的剂量依赖性，但AM产生的TNF明显多于血液单核细胞。单克隆抗TNF - α抗体完全抑制TNF活性。粒细胞-巨噬细胞集落刺激因子（GM - CSF）诱导单核细胞体外成熟产生的巨噬细胞比新鲜分离的单核细胞产生更多的TNF。在激活刺激后，来自健康供体和肺癌患者的AM产生TNF的能力没有差异。这些观察结果表明，人类AM可能通过TNF - α的产生在体内抗肿瘤防御中发挥重要作用。
The abilities of human alveolar macrophages (AM) obtained from healthy donors and patients with lung cancer to produce tumor necrosis factor (TNF) were compared with those of their blood monocytes after activation with lipopolysaccharide (LPS). TNF activity was assayed by measuring cytotoxicity against actinomycin D‐treated L929 cells and TNF was determined quantitatively by sandwich enzyme‐linked immnnosorbent assay (ELISA) with polyclonal and monoclonal antibodies against TNF‐α. Unstimulated AM from healthy donors released variable amounts of TNF spontaneously, whereas blood monocytes did not. When treated with LPS for 24 h, AM and monocytes produced TNF dose‐dependently, but TNF production by AM was significantly more than that by blood monocytes. This TNF activity was inhibited completely by monoclonal anti‐TNF‐α antibody. Macrophages generated by in vitro maturation of monocytes induced by granulocyte‐macrophage colony‐stimulating factor (GM‐CSF) produced more TNF than freshly isolated monocytes. No difference was found in the abilities of AM from healthy donors and patients with lung cancer to produce TNF after activation stimuli. These observations suggest that human AM may be important in in vivo antitumor defense of the lung through TNF‐α production.