Measuring non-radiative relaxation time of fluorophores with biomedical applications by intensity-modulated laser-induced photoacoustic effect.
Measuring non-radiative relaxation time of fluorophores with biomedical applications by intensity-modulated laser-induced photoacoustic effect.
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DOI：
10.1364/boe.2.002749
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发表时间：
2011-10-01
影响因子：
3.4
通讯作者：
Yang X
中科院分区：
文献类型：
--
作者：
Soroushian B;Yang X
利用调制猝发光的光声效应测量了具有重要生物医学意义的荧光团的非辐射弛豫时间。通过光声信号幅度的频率依赖性估计了非辐射弛豫时间。对新的吲哚菁绿色（IR-820）（其是近红外染料并且具有生物医学应用）在两种不同溶剂（水和二甲基亚砜（DMSO））中的溶液进行实验。与水溶液相比，染料在DMSO中的溶液的非辐射弛豫慢1.5倍。这一结果与三重态分子的非辐射弛豫依赖于其所溶解的溶剂的粘度这一普遍发现相一致。无辐射弛豫时间的测量可以作为光声成像技术中对比度机制的一个新来源。所提出的方法具有潜在的应用，如成像组织氧合和映射的其他化学物理差异的微环境中的外源性生物标志物。
Modulated tone-burst light was employed to measure non-radiative relaxation time of fluorophores with biomedical importance through photoacoustic effect. Non-radiative relaxation time was estimated through the frequency dependence of photoacoustic signal amplitude. Experiments were performed on solutions of new indocyanine green (IR-820), which is a near infrared dye and has biomedical applications, in two different solvents (water and dimethyl sulfoxide (DMSO)). A 1.5 times slower non-radiative relaxation for the solution of dye in DMSO was observed comparing with the aqueous solution. This result agrees well with general finding that non-radiative relaxation of molecules in triplet state depends on viscosity of solvents in which they are dissolved. Measurements of the non-radiative relaxation time can be used as a new source of contrast mechanism in photoacoustic imaging technique. The proposed method has potential applications such as imaging tissue oxygenation and mapping of other chemophysical differences in microenvironment of exogenous biomarkers.