Decorin-binding protein A (DbpA) of Borrelia burgdorferi is not protective when immunized mice are challenged via tick infestation and correlates with the lack of DbpA expression by B-burgdorferi in ticks
Decorin-binding protein A (DbpA) of Borrelia burgdorferi is not protective when immunized mice are challenged via tick infestation and correlates with the lack of DbpA expression by B-burgdorferi in ticks
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DOI：
10.1128/iai.68.8.4759-4764.2000
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发表时间：
2000-08-01
影响因子：
3.1
通讯作者：
Norgard, MV
中科院分区：
文献类型：
--
作者：
Hagman, KE;Yang, XF;Norgard, MV
以前的研究表明，核心蛋白聚糖结合蛋白A（DbpA）的伯氏疏螺旋体是一种保护性免疫原在小鼠模型莱姆病时，小鼠皮内攻击（针接种）与体外培养的螺旋体。在本研究中，DbpA免疫的C3 H/HeJ小鼠在感染携带强毒B的肩突硬蜱时不能免受感染。第297章.这种保护的缺乏与未能检测到B上的DbpA相关。提示当B.感染伯氏菌的蜱从免疫的宿主那里吸血。DbpA免疫保护蜱攻击小鼠的失败与早期针接种疫苗接种实验的结果相矛盾，并表明DbpA可能不适合作为莱姆病疫苗。
Previous studies showed that decorin-binding protein A (DbpA) of Borrelia burgdorferi was a protective immunogen in the murine model of Lyme borreliosis when mice were challenged (needle inoculated) intradermally with in vitro-cultivated spirochetes. In the present study, DbpA-immunized C3H/HeJ mice were not protected from infection when infested with Ixodes scapularis nymphs harboring virulent B. burgdorferi 297. This lack of protection correlated with the failure to detect DbpA on B. burgdorferi in ticks, suggesting that DbpA is not available as a target for bactericidal antibodies in serum when B. burgdorferi-infected ticks take their blood meal from an immunized host. The failure of DbpA immunization to protect tick-challenged mice contradicts the results of earlier needle inoculation vaccination experiments and suggests that DbpA may not be suitable as a Lyme disease vaccine.