Quantitative analysis of the experimental O-J-I-P chlorophyll fluorescence induction kinetics
Quantitative analysis of the experimental O-J-I-P chlorophyll fluorescence induction kinetics
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DOI：
10.1111/j.1742-4658.2006.05475.x
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发表时间：
2006-10-01
期刊：
影响因子：
5.4
通讯作者：
Carpentier, Robert
中科院分区：
文献类型：
--
作者：
Boisvert, Steve;Joly, David;Carpentier, Robert
荧光诱导已经研究了很长时间，但仍有问题，关于什么是O-J-I-P动力学步骤代表。大多数研究认为O-J升高与光系统II初级受体（Q（A））减少有关，但对于J-I和I-P升高存在一些相互矛盾的理论。荧光诱导分析的一个问题是，迄今为止所做的大多数工作仅使用定性或半定量数据分析，通过视觉比较痕迹来观察不同化学品或处理的效果。虽然这种方法对于观察主要变化是有用的，但必须使用定量方法来检测荧光诱导痕迹中更细微但重要的差异。为了实现这一点，我们使用了一个相对简单的数学方法来提取的幅度和半衰期的三个主要的荧光诱导阶段获得的痕迹在类囊体膜保持在不同的温度下测量。还获得了每个动力学步骤的表观活化能（E-A）。结果表明，各相具有不同的E-A值，EAO-J <EAJ-I <EAI-P，因此各相的成因不同。两个众所周知的化学品，3-（3，4-二氯苯基）-1，1-二甲基脲，它阻止电子转移到光系统II的二次电子受体（Q（B）），和decylplastoquinone，它的行为类似于内源性可还原plastoquinones，对定量参数的影响进行了讨论，在每个动力学阶段的起源。
Fluorescence induction has been studied for a long time, but there are still questions concerning what the O-J-I-P kinetic steps represent. Most studies agree that the O-J rise is related to photosystem II primary acceptor (Q(A)) reduction, but several contradictory theories exist for the J-I and I-P rises. One problem with fluorescence induction analysis is that most work done to date has used only qualitative or semiquantitative data analysis by visually comparing traces to observe the effects of different chemicals or treatments. Although this method is useful to observe major changes, a quantitative method must be used to detect more subtle, yet important, differences in the fluorescence induction trace. To achieve this, we used a relatively simple mathematical approach to extract the amplitudes and half-times of the three major fluorescence induction phases obtained from traces measured in thylakoid membranes kept at various temperatures. Apparent activation energies (E-A) were also obtained for each kinetic step. Our results show that each phase has a different E-A, with EA O-J < EA J-I < EA I-P, and thus a different origin. The effects of two well-known chemicals, 3-(3,4-dichlorophenyl)-1,1-dimethylurea, which blocks electron transfer to the photosystem II secondary electron acceptor (Q(B)), and decylplastoquinone, which acts similarly to endogenous reducible plastoquinones, on the quantitative parameters are discussed in terms of the origin of each kinetic phase.