Immunochromatographic assay for quantitation of milk progesterone
Immunochromatographic assay for quantitation of milk progesterone
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DOI：
10.3891/acta.chem.scand.50-0141
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发表时间：
1996-02-01
期刊：
影响因子：
--
通讯作者：
Vuento, M
中科院分区：
文献类型：
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作者：
Laitinen, MPA;Vuento, M
我们描述了一种快速免疫层析定量牛乳中黄体酮的方法。该方法基于“竞争性”分析格式，使用黄体酮单克隆抗体和用胶体金颗粒标记的黄体酮-蛋白偶联物。该单克隆抗体是作为一个狭窄的检测区固定在多孔膜上。将样品与涂有孕酮蛋白偶联物的胶体金颗粒混合，并允许混合物迁移过检测区。毛细管力促进了迁移。通过光度扫描检测到的与检测区结合的标记黄体酮蛋白偶联物的量与样品中存在的黄体酮的量成反比。分析在不到10分钟内完成。该方法的实际检出限为每毫升牛乳5 ng黄体酮。
We describe a rapid immunochromatographic method for the quantitation of progesterone in bovine milk. The method is based on a 'competitive' assay format using the monoclonal antibody to progesterone and a progesterone-protein conjugate labelled with colloidal gold particles. The monoclonal antibody to proges terone is immobilized as a narrow detection zone on a porous membrane. The sample is mixed with colloidal gold particles coated with progesterone-protein conjugate, and the mixture is allowed to migrate past the detection zone. Migration is facilitated by capillary forces. The amount of labelled progesterone-protein conjugate bound to the detection zone, as detected by photometric scanning, is inversely proportional to the amount of progesterone present in the sample. Analysis is complete in less that 10 min. The method has a practical detection limit of 5 ng of progesterone per mi of bovine milk.