Microscope-based near-infrared stereo-imaging system for quantifying the motion of the murine epicardial coronary arteries in vivo.
Microscope-based near-infrared stereo-imaging system for quantifying the motion of the murine epicardial coronary arteries in vivo.
复制标题
基于显微镜的近红外立体成像系统，用于量化体内小鼠心外膜冠状动脉的运动。
DOI：
10.1117/1.jbo.18.9.096013
复制
发表时间：
2013
影响因子：
3.5
通讯作者：
Friedman,MortonH
中科院分区：
文献类型：
--
作者：
Long,DavidS;Zhu,Hui;Friedman,MortonH
动脉粥样硬化是工业化国家死亡的主要原因。除了动脉粥样硬化的“传统”全身性危险因素外，冠状动脉的几何形状和运动可能有助于个体对这些血管中疾病的发展和进展的易感性。为了能够测试这一点，我们已经开发了一个高速（每秒帧数）显微镜为基础的立体成像系统，以量化小鼠心外膜冠状动脉的运动。使用近红外非靶向量子点作为成像对比剂，我们同步采集了手术暴露的小鼠心脏的成对图像，从中重建了冠状动脉的三维几何形状。通过心动周期逐帧跟踪重建的几何形状，以量化血管的体内运动，从中导出位移、曲率和扭转参数。给出了C57 BL/6 J小鼠的说明性结果。
Atherosclerosis is a leading cause of mortality in industrialized countries. In addition to “traditional” systemic risk factors for atherosclerosis, the geometry and motion of coronary arteries may contribute to individual susceptibility to the development and progression of disease in these vessels. To be able to test this, we have developed a high-speed (frames per second) microscope-based stereo-imaging system to quantify the motion of epicardial coronary arteries of mice. Using near-infrared nontargeted quantum dots as an imaging contrast agent, we synchronously acquired paired images of a surgically exposed murine heart, from which the three-dimensional geometry of the coronary arteries was reconstructed. The reconstructed geometry was tracked frame by frame through the cardiac cycle to quantify thein vivomotion of the vessel, from which displacements, curvature, and torsion parameters were derived. Illustrative results for a C57BL/6J mouse are presented.