A positional Toll receptor code directs convergent extension in Drosophila.
A positional Toll receptor code directs convergent extension in Drosophila.
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DOI：
10.1038/nature13953
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发表时间：
2014-11-27
期刊：
影响因子：
64.8
通讯作者：
Zallen JA
中科院分区：
文献类型：
--
作者：
Paré AC;Vichas A;Fincher CT;Mirman Z;Farrell DL;Mainieri A;Zallen JA
通过会聚延伸的头到尾体轴的延长是整个后生动物保守的发育过程。在果蝇中，转录因子的表达模式提供了空间线索，诱导系统定向的细胞运动，促进组织伸长。然而，模式化的转录输入控制细胞极性和行为的机制一直是难以捉摸的。我们证明，三个Toll家族受体，Toll-2，Toll-6，和Toll-8，表示在重叠的横向条纹沿着前后轴和行动相结合，以指导平面极性和极化细胞重排在收敛扩展。同时中断所有三种受体强烈降低肌动球蛋白驱动的连接重塑和轴伸长，和Toll受体表达的异位条纹足以诱导平面极化肌动球蛋白收缩。这些结果表明，Toll受体表达的组织水平模式提供了空间信号，将来自前后图案化系统的位置信息与驱动会聚延伸的基本细胞行为联系起来。
Elongation of the head-to-tail body axis by convergent extension is a conserved developmental process throughout metazoans. In Drosophila, patterns of transcription factor expression provide spatial cues that induce systematically oriented cell movements and promote tissue elongation. However, the mechanisms by which patterned transcriptional inputs control cell polarity and behavior have long been elusive. We demonstrate that three Toll family receptors, Toll-2, Toll-6, and Toll-8, are expressed in overlapping transverse stripes along the anterior-posterior axis and act in combination to direct planar polarity and polarized cell rearrangements during convergent extension. Simultaneous disruption of all three receptors strongly reduces actomyosin-driven junctional remodeling and axis elongation, and an ectopic stripe of Toll receptor expression is sufficient to induce planar polarized actomyosin contractility. These results demonstrate that tissue-level patterns of Toll receptor expression provide spatial signals that link positional information from the anterior-posterior patterning system to the essential cell behaviors that drive convergent extension.