Disruption of the gene encoding the V-ATPase subunit A results in inhibition of normal growth and abolished sporulation in Aspergillus nidulans
Disruption of the gene encoding the V-ATPase subunit A results in inhibition of normal growth and abolished sporulation in Aspergillus nidulans
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DOI：
10.1099/mic.0.26807-0
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发表时间：
2004-03-01
期刊：
影响因子：
2.8
通讯作者：
Wagner, EGH
中科院分区：
文献类型：
--
作者：
Melin, P;Schnürer, J;Wagner, EGH
作者先前报道了构巢曲霉对细菌抗真菌代谢物的分子反应，例如巴非霉素和相关的康卡那霉素。这些化合物是已知的V-ATP酶抑制剂，并对菌丝体生长和形态产生显著影响。在粗糙脉孢菌中，研究表明，破坏编码V-ATP酶亚基A的基因会导致形态变化和生长减少，这与添加康卡霉素后观察到的情况相似。这种表型，以及这种突变赋予对康卡霉素的抗性的事实，表明V-ATP酶是抗生素的主要（或唯一）靶标。然而，生长抑制和形态变化，例如，A。nidulans和Penicilliumroqueforti更严重，因此其他靶标也是可能的。本研究中，将A.通过同源重组破坏了编码V-ATPase亚基A的nidulans。得到的vmaA 1突变株显示出极慢的生长并且不能产生无性孢子。此外，观察到与添加V-ATP酶抑制剂（即巴夫洛霉素或康卡霉素）引起的形态学改变相似的形态学改变，表明V-ATP酶也是A. nidulans。vmaA 1突变体在pH值高于7时不能存活，对高浓度Zn 2+高度敏感，这与酿酒酵母（Saccharomyces cerevisiae）和N.粗鲁。
The authors have previously reported on molecular responses of Aspergillus nidulans to bacterial antifungal metabolites, e.g. bafilomycins and the related concanamycins. These compounds are known inhibitors of V-ATPases and cause dramatic effects on mycelial growth and morphology. In Neurospora crassa, studies have shown that disruption of the gene encoding subunit A of the V-ATPase results in morphological changes and reduced growth similar to those observed after addition of concanamycin. This phenotype, and the fact that this mutation confers resistance to concanamycin, suggests that V-ATPase is the main (or only) target for the antibiotics. However, growth inhibition and morphology changes in, for example, A. nidulans and Penicillium roqueforti are more severe, and thus other targets are possible. In this study, the vmaA gene of A. nidulans, encoding the subunit A of V-ATPase, was disrupted by homologous recombination. The resulting vmaA 1 mutant strain displayed extremely slow growth and failed to produce asexual spores. Furthermore, an altered morphology similar to that caused by addition of V-ATPase inhibitors, i.e. bafilomycin or concanamycin, was observed, indicating that V-ATPase is the main target for the antibiotics also in A. nidulans. The vmaA 1 mutant was not viable at pH values above 7 and was highly sensitive to high Zn2+ concentrations, in agreement with previous results from studies of Saccharomyces cerevisiae and N. crassa.