ORIGIN, DIFFERENTIATION AND RENEWAL OF 4 MAIN EPITHELIAL-CELL TYPES IN MOUSE SMALL INTESTINE .4. PANETH CELLS
ORIGIN, DIFFERENTIATION AND RENEWAL OF 4 MAIN EPITHELIAL-CELL TYPES IN MOUSE SMALL INTESTINE .4. PANETH CELLS
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DOI：
10.1002/aja.1001410406
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发表时间：
1974-01-01
影响因子：
--
通讯作者：
CHENG, H
中科院分区：
文献类型：
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作者：
CHENG, H
单次注射3h胸腺嘧啶后，用电镜放射自显像法观察成年小鼠小肠Paneth细胞的起源、分化和命运。Paneth细胞群由23%的年轻Paneth细胞组成，颗粒直径小于2 μm， 63%的成熟Paneth细胞颗粒直径为2 - 3 μm， 14%的老Paneth细胞颗粒直径大于3 μm。光镜和电镜放射自显像证实，典型的Paneth细胞在注射3h胸苷1小时后未被标记，因此不能增殖。然而，在注射后12小时，第一次观察到带有少量Paneth细胞颗粒的标记细胞。除了颗粒外，这些细胞与高度增殖的隐窝基柱状细胞难以区分。因此，我们怀疑Paneth细胞来源于隐窝基柱状细胞。注射后随着时间的推移，分化继续进行：出现粗糙的内质网池，颗粒逐渐增多和变大，核获得不规则形状。注射后5天观察标记的成熟Paneth细胞。与其他三种细胞类型不同，Paneth细胞不会向上迁移到绒毛上皮，因此不会通过挤压区丢失。相反，它们在隐窝基部发生变性，最终被邻近的隐窝基部柱状细胞吞噬。
The origin, differentiation and fate of Paneth cells in adult mouse small intestine was examined by electron microscopic radioautography at various times after a single injection of3H‐thymidine.The Paneth cell population consists of 23%youngPaneth cells with granules less than 2 μm in diameter, 63%maturePaneth cells with granules 2–3 μm in diameter, and 14%oldPaneth cells with granules greater than 3 μm in diameter.Light and electron microscopic radioautography confirms that typical Paneth cells are not labeled 1 hour after3H‐thymidine injection and, therefore, are not capable of proliferation. However, labeled cells with a few small Paneth cell granules are first observed 12 hours after an injection. Except for the presence of granules, these cells are indistinguishable from the highly proliferative crypt‐base columnar cells. It is, therefore, suspected that Paneth cells are derived from crypt‐base columnar cells.With time after injection, differentiation proceeds: stacks of rough endoplasmic reticulum cisternae appear, the granules become progressively more numerous and larger, and the nucleus acquires an irregular shape. Labeled mature Paneth cells are observed by five days after injection.Unlike the other three cell types, Paneth cells do not migrate up to the villus epithelium and thus are not lost through the extrusion zone. Instead they undergo degeneration in the crypt‐base and are eventually phagocytosed by an adjacent crypt‐base columnar cell.