Comparative expression profiling of 40 mouse cytochrome P450 genes in embryonic and adult tissues
Comparative expression profiling of 40 mouse cytochrome P450 genes in embryonic and adult tissues
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DOI：
10.1016/s0003-9861(03)00174-7
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发表时间：
2003-06-01
影响因子：
3.9
通讯作者：
Stoilov, I
中科院分区：
文献类型：
--
作者：
Choudhary, D;Jansson, I;Stoilov, I
这项研究是第一个系统的调查孕龄依赖性和成人组织特异性表达模式的每个已知的小鼠hepatitis B家族（40个基因）使用归一化的cDNA面板和统一的逆转录酶聚合酶链反应为基础的测定。27个P450在发育过程中组成型表达。在原肠胚形成期E7（n = 14）、神经图式和体节形成期E11（n = 17）、器官形成期E15（n = 20）和胎儿期E17（n = 21），这些数目逐渐增加。Cyp2s1、Cyp8a1、Cyp20、Cyp21a1、Cyp26a1、Cyp46和Cyp51在所研究的四个阶段中的每一个中被检测到。CYP1家族成员表现出复杂的、非重叠的胚胎表达模式，表明Cyp1a1和Cyp1a2可能不能补偿与异常眼发育相关的Cyp1b1缺陷。发现多种Cyp形式在所研究的每个成人组织中组成型表达：肝（n = 31）、肾（n = 30）、睾丸（n = 26）、肺（n = 24）和心脏（n = 13）。本研究中报道的组织特异性P450表达谱为更集中地分析细胞色素P450单加氧酶的组织特异性和发育功能提供了参考。(C)2003 Elsevier Science（美国）。All rights reserved.
This study is the first systematic investigation of the gestational age-dependent and adult tissue-specific expression patterns of each known mouse CYP family (40 genes) using normalized cDNA panels and uniform reverse transcriptase polymerase chain reaction-based assays. Twenty-seven of the P450s were constitutively expressed during development. The number gradually increased through the phases of gastrulation E7 (n = 14), neural patterning and somitogenesis E I I (n = 17), organogenesis El 5 (n = 20), and fetal period E17 (n = 21). Cyp2s1, Cyp8a1, Cyp20, Cyp21a1, Cyp26a1, Cyp46, and Cyp51 were detected in each of the four stages studied. Members of family CYP1 demonstrated complex, nonoverlapping embryonic patterns of expression, indicating that Cyp1a1 and Cyp1a2 may not compensate for Cyp1b1 deficiency associated with abnormal eye development. Multiple Cyp forms were found to be constitutively expressed in each of the adult tissues studied: liver (n = 31), kidney (n = 30), testis (n = 26), lung (n = 24), and heart (n = 13). The tissue-specific P450-expression profiles reported in this study provide a reference for more focused analysis of the tissue-specific and developmental functions of the cytochrome P450 monooxygenases. (C) 2003 Elsevier Science (USA). All rights reserved.