ETTIN patterns the Arabidopsis floral meristem and reproductive organs.
ETTIN patterns the Arabidopsis floral meristem and reproductive organs.
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发表时间：
1997-11
期刊：
影响因子：
4.6
通讯作者：
Allen Sessions;Jennlfer L. Nemhauser;Andy McColl;Andy McColl;Judith L. Roe;Kenneth A. Feldmann
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文献类型：
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作者：
Allen Sessions;Jennlfer L. Nemhauser;Andy McColl;Andy McColl;Judith L. Roe;Kenneth A. Feldmann
Ettin(Ett)突变对拟南芥的花发育具有多效性，导致花被器官数量增加，雄蕊数量和花药形成减少，雌蕊群顶端-基端模式缺陷。ETTIN基因被克隆并编码一种与DNA结合蛋白同源的蛋白，该蛋白与生长素反应元件结合。ETT转录本在第1阶段花分生组织中表达，随后在花瓣、雄蕊和心皮原基中分解成复杂的模式。这些数据表明，ETT在花分生组织中传递区域特性，从而影响花被器官数间距、雄蕊形成以及雄蕊和雌蕊的区域分化。在第5期，ETT的表达在雌蕊群形态出现之前出现在花分生组织顶端的环状区域，这与ETT参与了雌蕊原基顶端和基端边界的形成的观点一致。双突变分析和表达研究表明，尽管ETT的转录激活独立于分生组织和器官识别基因LEAFY、APETELA1、APETELA2和AGAMOUS，但这些基因的功能是ETT活性所必需的。双突变分析还表明，ETT的功能独立于‘b’类基因APETELA3和pistillata。最后，双突变分析表明，ETT对花器官数量的控制独立于CLAVATA基因座，与PERIANTHIA基因冗余。
ettin (ett) mutations have pleiotropic effects on Arabidopsis flower development, causing increases in perianth organ number, decreases in stamen number and anther formation, and apical-basal patterning defects in the gynoecium. The ETTIN gene was cloned and encodes a protein with homology to DNA binding proteins which bind to auxin response elements. ETT transcript is expressed throughout stage 1 floral meristems and subsequently resolves to a complex pattern within petal, stamen and carpel primordia. The data suggest that ETT functions to impart regional identity in floral meristems that affects perianth organ number spacing, stamen formation, and regional differentiation in stamens and the gynoecium. During stage 5, ETT expression appears in a ring at the top of the floral meristem before morphological appearance of the gynoecium, consistent with the proposal that ETT is involved in prepatterning apical and basal boundaries in the gynoecium primordium. Double mutant analyses and expression studies show that although ETT transcriptional activation occurs independently of the meristem and organ identity genes LEAFY, APETELA1, APETELA2 and AGAMOUS, the functioning of these genes is necessary for ETT activity. Double mutant analyses also demonstrate that ETT functions independently of the 'b' class genes APETELA3 and PISTILLATA. Lastly, double mutant analyses suggest that ETT control of floral organ number acts independently of CLAVATA loci and redundantly with PERIANTHIA.