Flow cytometric quantification and immunophenotyping of leukemic stem cells in acute myeloid leukemia
Flow cytometric quantification and immunophenotyping of leukemic stem cells in acute myeloid leukemia
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DOI：
10.1007/s00277-012-1501-7
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发表时间：
2012-10-01
影响因子：
3.5
通讯作者：
Seo, Jong-Jin
中科院分区：
文献类型：
--
作者：
Hwang, Keumrock;Park, Chan-Jeoung;Seo, Jong-Jin
白血病干细胞（LSC）是急性髓系白血病（AML）克隆生长的根源，负责白血病原始细胞（LB）的增殖。LSC被认为是LB中的CD 34 + CD 38-群体，通常表达CD 123，CD 44或CD 184，这些在正常造血干细胞上很少表达，可能是潜在的治疗靶点。采用多色流式细胞术分析了63例AML患者中CD 34 + CD 38-LSC的比例以及LSC上CD 123、CD 44和CD 184的表达。诊断时LSC的中位比例为1.3%（0.0- 33.1%）。在所有患者中，分别有74.6%的患者具有CD 123阳性LSC，所有患者具有CD 44阳性LSC，85.7%具有CD 184阳性LSC。完全缓解（CR）组LSC的比例显著低于非CR组（P = 0.006）。CR组LSC比例较低，提示检测LSC比例有助于判断AML的预后。
Leukemic stem cells (LSCs) are root of clonal growth in acute myeloid leukemia (AML) and responsible for the propagation of leukemic blasts (LBs). LSCs are considered as CD34 + CD38- population among LBs and often express as CD123, CD44, or CD184, which are rarely expressed on normal hematopoietic stem cells and could be the potential therapeutic targets. Using multi-color flow cytometry, we analyzed the proportions of CD34 + CD38- LSCs and expression of CD123, CD44, and CD184 on LSCs in 63 patients with AML. The median proportion of LSCs was 1.3 % (0.0-33.1 %) at the time of diagnosis. Of all patients, 74.6 % of them had CD123-positive LSCs, all patients had CD44-positive LSCs, and 85.7 % had CD184-positive LSCs, respectively. The proportions of LSCs were significantly lower in the complete remission (CR) group compared with non-CR group (P = 0.006). The lower proportions of LSCs in CR group indicated that measurement of the proportion of LSCs might be helpful to predict the prognosis of AML.