Proteomic profiling of halloysite clay nanotube exposure in intestinal cell co-culture
Proteomic profiling of halloysite clay nanotube exposure in intestinal cell co-culture
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DOI：
10.1002/jat.2858
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发表时间：
2013-11-01
影响因子：
3.3
通讯作者：
Witzmann, Frank A.
中科院分区：
文献类型：
--
作者：
Lai, Xianyin;Agarwal, Mangilal;Witzmann, Frank A.
埃洛石是具有纳米级内径和外径的中空管状结构的铝硅酸盐粘土。鉴于埃洛石在口服给药中的潜在应用，其生物相容性评价变得重要。为了研究埃洛石纳米管对大肠体外模型的影响，将单层共培养的Caco-2/HT 29-MTX细胞暴露于纳米管进行毒性测试和蛋白质组学分析。结果表明，埃洛石表现出高度的生物相容性，其特征在于没有细胞毒性，尽管升高的促炎细胞因子释放。在分析的4081种蛋白质中观察到表达的暴露特异性变化。差异表达蛋白质谱的生物信息学分析表明，埃洛石刺激与细胞生长和增殖，细胞感染，刺激和损伤的微妙反应，增强抗氧化能力，以及对暴露的整体适应性反应相关的过程。这些潜在的相关功能效应需要在体内模型中进一步研究，并表明长期或大剂量职业暴露于埃洛石纳米管可能会产生意想不到的结果。版权所有（c）2013约翰威利父子有限公司
Halloysite is aluminosilicate clay with a hollow tubular structure with nanoscale internal and external diameters. Assessment of halloysite biocompatibility has gained importance in view of its potential application in oral drug delivery. To investigate the effect of halloysite nanotubes on an in vitro model of the large intestine, Caco-2/HT29-MTX cells in monolayer co-culture were exposed to nanotubes for toxicity tests and proteomic analysis. Results indicate that halloysite exhibits a high degree of biocompatibility characterized by an absence of cytotoxicity, in spite of elevated pro-inflammatory cytokine release. Exposure-specific changes in expression were observed among 4081 proteins analyzed. Bioinformatic analysis of differentially expressed protein profiles suggest that halloysite stimulates processes related to cell growth and proliferation, subtle responses to cell infection, irritation and injury, enhanced antioxidant capability, and an overall adaptive response to exposure. These potentially relevant functional effects warrant further investigation in in vivo models and suggest that chronic or bolus occupational exposure to halloysite nanotubes may have unintended outcomes. Copyright (c) 2013 John Wiley & Sons, Ltd.