Cytoplasmic polarity develops at compaction in rat and mouse embryos.
Cytoplasmic polarity develops at compaction in rat and mouse embryos.
复制标题
大鼠和小鼠胚胎压缩时细胞质极性形成。
DOI：
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发表时间：
1981
期刊：
影响因子：
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通讯作者：
By W. J. D. Reeve
中科院分区：
文献类型：
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作者：
By W. J. D. Reeve
大鼠和小鼠桑椹胚的细胞都可以被染色，以显示其细胞质组织的不对称性。甲苯胺蓝、吖啶橙子和辣根过氧化物酶（HRP）显示，在大鼠8-细胞胚胎的每个细胞中，细胞核和胚胎外周之间形成一列细胞器。虽然小鼠桑椹胚细胞缺乏明显的不对称分布的细胞器中观察到的大鼠细胞，长脉冲（大于3小时）的HRP紧凑的8细胞小鼠胚胎显示了一个明显的限制本地化的酶不明显在早期的预压实阶段。这些胚胎中产生的细胞质极性可以在完整胚胎的细胞中证明，也可以在活体染色前从胚胎中分解的细胞中证明。
Cells of both rat and mouse morulae can be stained vitally to reveal an asymmetry in the organization of their cytoplasm. In each cell of the rat 8-cell-embryo a column of organelles develops between the nucleus and the embryo periphery as revealed by toluidine blue, acridine orange and horseradish peroxidase (HRP). Although cells of the mouse morula lack the blatant asymmetric distribution of organelles observed in rat cells, a long pulse (greater than 3 h) of HRP to compact 8-cell mouse embryos revealed a distinct restricted localization of the enzyme not evident at earlier pre-compaction stages. The cytoplasmic polarity generated in these embryos can be demonstrated in cells of intact embryos, and also in cells disaggregated from embryos before vital staining.