In situ real-time investigation of cancer cell photothermolysis mediated by excited gold nanorod surface plasmons
In situ real-time investigation of cancer cell photothermolysis mediated by excited gold nanorod surface plasmons
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DOI：
10.1016/j.biomaterials.2010.01.140
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发表时间：
2010-05-01
期刊：
影响因子：
14
通讯作者：
Chen, Yang-Yuan
中科院分区：
文献类型：
--
作者：
Chen, Cheng-Lung;Kuo, Ling-Ru;Chen, Yang-Yuan
采用金纳米棒（AuNRs）双光子照射，对EMT-6乳腺癌细胞进行了原位光热照射，并进行了实时观察。细胞的形态和质膜通透性是这种现象的关键指标。采用种子介导法合成了长径比为3.92、在300 nm处有纵向吸收峰的金核受体。纳米棒表面进一步改性聚苯乙烯磺酸盐（PSS）的生物相容性。所制备的纳米棒显示出优异的双光子光致发光成像。原位实时结果显示，细胞内部的空腔是由AuNRs局部光热效应引发的热爆炸产生的。空化动力学是能量依赖性的，并负责质膜的穿孔或突然破裂。细胞治疗的能量阈值显著依赖于每个细胞所吸收的纳米棒的数量。对于类似于每个细胞10-30个的摄入AuNR团簇数量N，发现能量注量E大于93 mJ/cm（2）导致在非常短的时间内以破碎形式有效地破坏细胞。对于较低能级E = 18 mJ/cm（2），N类似于60-100，观察到非瞬时但进行性的电池劣化。(C)2010爱思唯尔有限公司版权所有。
The photothermolysis of living EMT-6 breast tumor cells triggered by gold nanorods (AuNRs) with two-photon irradiation was conducted in situ and under real-time observation. The morphology and plasma membrane permeability of the cells were key indicators to the phenomena. AuNRs with an aspect ratio of 3.92, and a longitudinal absorption peak at 300 nm were synthesized with a seed-mediated method. The nanorods surfaces were further modified with polystyrenesulfonate (PSS) for biocompatibility. The prepared nanorods displayed excellent two-photon photoluminescence imaging. In situ real-time results revealed cavities internal to the cells were created from thermal explosions triggered by AuNRs localized photothermal effect. The cavitation dynamic is energy dependent and responsible for the perforation or sudden rupture of the plasma membrane. The energy threshold for cell therapy depended significantly on the number of nanorods taken up per cell. For an ingested AuNR cluster quantity N similar to 10-30 per cell, it is found that energy fluences E larger-than 93 mJ/cm(2) led to effective cell destruction in the crumbled form within a very short period. As for a lower energy level E = 18 mJ/cm(2) with N similar to 60-100, a non-instant, but progressive cell deterioration, is observed. (C) 2010 Elsevier Ltd. All rights reserved.