Membrane asymmetry enhances nanotube formation and limits pore resealing after electroporation
Membrane asymmetry enhances nanotube formation and limits pore resealing after electroporation
复制标题
膜的不对称性增强了纳米管的形成并限制了电穿孔后孔的重新密封
DOI：
10.1016/j.bpj.2022.07.037
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发表时间：
2022
影响因子：
3.4
通讯作者：
Kelly, Christopher V.
中科院分区：
文献类型：
--
作者：
Gandhi, Sonali A.;Kelly, Christopher V.
细胞膜研究为自然世界、生物工程和基础物理学提供了一个迷人的交叉点。模型膜是由两亲性磷脂自组装成两分子厚的流体片，跨度可达数十微米。越来越多的模型膜包括小叶不对称、脂相分离和自发曲率，从而能够检查各种物理特性的相互作用，这些特性可能控制复杂的自然行为。例如，脂质相分离和小叶不对称都促进膜曲率的形成，但活细胞利用这些关系的程度尚不清楚。悬而未决的问题仍然是如何组成的异质性，形状和动力学耦合，以减少外部能源的需求（即ATP）在复杂的行为表现。进一步的研究需要了解细胞如何调节其高度动态和精确控制的膜形状，同时确保膜的完整性。Aleksanyan等人在本期生物物理杂志(1)上报道的结果详细说明了小叶不对称-
Research in cell membranes provides a fascinating intersection of the natural world, bioengineering, and fundamental physics. Model membranes form by the self-assembly of amphiphilic phospholipids into two-molecule-thick fluid sheets spanning up to tens of microns. Increasingly, model membranes include leaflet asymmetry, lipid phase separation, and spontaneous curvature to enable examination of the interplay of diverse physical characteristics that potentially govern complex natural behaviors. For example, lipid phase separation and leaflet asymmetry both encourage the formation of membrane curvature, but the degree to which living cells exploit these relationships is unknown.Outstanding questions remain regarding how compositional heterogeneity, shape, and dynamics couple to reduce the demand for external energy sources (ie, ATP) during the performance of complex behaviors. Further research is required to understand how cells regulate their highly dynamic and precisely controlled membrane shapes while ensuring membrane integrity. The results by Aleksanyan et al. reported in this issue of the Biophysical Journal (1) detail how leaflet asymme-