Designing an anti-inflammatory and tissue-adhesive colloidal dressing for wound treatment
Designing an anti-inflammatory and tissue-adhesive colloidal dressing for wound treatment
复制标题
DOI：
10.1016/j.colsurfb.2019.110737
复制
发表时间：
2020-04-01
影响因子：
5.8
通讯作者：
Taguchi, Tetsushi
中科院分区：
文献类型：
--
作者：
Nishiguchi, Akihiro;Taguchi, Tetsushi
创面敷料广泛用于保护创面不受外界环境影响，促进创面愈合。然而，传统的伤口敷料缺乏组织黏附性和抗炎功能，导致诸如内镜手术后胃肠道伤口的纤维化和狭窄。在目前的研究中，我们报告了由皮质类固醇修饰的明胶颗粒组成的伤口敷料的组织粘附和抗炎特性。以氢化可的松（HC）为一类抗炎皮质类固醇，对阿拉斯加鳕鱼明胶（ApGltn）进行修饰，合成HC修饰的ApGltn （HC-ApGltn）。在HC-ApGltn水溶液中加入乙醇，在不使用有毒表面活性剂和交联试剂的情况下进行热交联（TC），制备了HC-ApGltn微颗粒（MPs）。用含胆固醇骨架结构的疏水HC修饰ApGltn，由于疏水相互作用，提高了ApGltn在潮湿条件下与胃粘膜下组织的粘附强度。在潮湿的条件下，这种粘接性能的保留可以稳定地保护伤口免受外部环境的影响。我们发现HC-ApGltn MPs被巨噬细胞摄取，并能有效抑制lps激活的巨噬细胞的形态学变化和炎症细胞因子的表达水平。强力的组织黏合剂和抗炎MPs可作为一种高级伤口敷料，保护伤口，抑制炎症反应，促进伤口愈合。
Wound dressing materials are widely used to protect wounds from the external environment and to promote wound healing. However, conventional wound dressings lack tissue adhesive properties and anti-inflammatory functions, which lead to fibrosis and stricture, in cases such as gastrointestinal wounds after endoscopic surgery. In the current study, we report tissue-adhesive and anti-inflammatory properties of a wound dressing composed of corticosteroid-modified gelatin particles. Hydrocortisone (HC), which is a class of anti-inflammatory corticosteroid, was used to modify Alaska-pollock gelatin (ApGltn) to synthesize HC-modified ApGltn (HC-ApGltn). Microparticles (MPs) of HC-ApGltn were fabricated by adding ethanol in HC-ApGltn aqueous solution and performing thermal crosslinking (TC) without the use of toxic surfactants and crosslinking reagents. Modification of ApGltn with hydrophobic HC containing cholesterol backbone structure improved its adhesion strength to gastric submucosal tissues under wet conditions owing to hydrophobic interactions. This retention of adhesive property under wet conditions allows for stable protection of wounds from the external environment. We found that HC-ApGltn MPs were taken up by macrophages and they effectively suppressed morphological changes of LPS-activated macrophages and the expression level of the inflammatory cytokine. Robust tissue adhesive and anti-inflammatory MPs may serve as an advanced wound dressing that can protect wounds and suppress inflammatory responses for promoting wound healing.