Parvalbumin-containing chandelier and basket cell boutons have distinctive modes of maturation in monkey prefrontal cortex.
Parvalbumin-containing chandelier and basket cell boutons have distinctive modes of maturation in monkey prefrontal cortex.
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DOI：
10.1523/jneurosci.0306-13.2013
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发表时间：
2013-05-08
期刊：
影响因子：
--
通讯作者：
Lewis DA
中科院分区：
文献类型：
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作者：
Fish KN;Hoftman GD;Sheikh W;Kitchens M;Lewis DA
含有小白蛋白（PV）的皮质GABA神经元包括枝形细胞（PVChCs）和篮状细胞（PVBCs），它们分别支配锥体细胞的轴突起始段（AIS）和索马体/近端树突。在猴前额叶皮层（PFC），密度的PVChC轴突盒检测PV免疫反应性的峰值之前，青春期的发病显着下降到成人的水平，而密度的PV免疫反应（IR）斑点（假定PVBC扣）在青春期增加。这些反向发展的变化，在扣接密度可以解释为什么电子显微镜研究发现没有变化的对称，大概GABA能，突触之间的婴儿期和成年期的猴PFC。或者，反向发展轨迹的PVChC和PVBC扣接可以代表PV蛋白水平的成熟细胞类型特异性差异。为了区分这两种选择，多标记共聚焦显微镜被用来量化的PVChC和PVBC扣每锥体神经元在PFC的3个月大和成年猴的数量。每个锥体神经元AIS的PVChC终扣的平均数量在成年猴中显著低于3个月龄猴的32%，而每个锥体神经元的PVBC终扣的密度在年龄组之间没有差异。相比之下，PV蛋白的相对水平在成年动物的PVBC扣约高出两倍，而PV水平在PVChC扣没有不同的年龄组。这些发现表明，猴PFC中含有PV的GABA能终扣成熟的细胞类型特异性机制。
Parvalbumin (PV)-containing cortical GABA neurons include chandelier cells (PVChCs) and basket cells (PVBCs), which innervate the axon initial segment (AIS) and soma/proximal dendrites of pyramidal cells, respectively. In monkey prefrontal cortex (PFC), the density of PVChC axon cartridges detectable by PV immunoreactivity peaks prior to the onset of puberty before declining markedly to adult levels, whereas the density of PV-immunoreactive (IR) puncta (presumed PVBC boutons) increases during adolescence. These inverse developmental changes in bouton density could explain why an electron microscopy study found no change in the density of symmetric, presumably GABAergic, synapses between infancy and adulthood in monkey PFC. Alternatively, the inverse developmental trajectories of PVChC and PVBC boutons could represent cell type-specific differences in the maturation of PV protein levels. To differentiate between these two alternatives, multilabel confocal microscopy was used to quantify the number of PVChC and PVBC boutons per pyramidal neuron in the PFC of 3-month-old and adult monkeys. The mean number of PVChC boutons per pyramidal neuron AIS was, significantly, 32% lower in adult compared with 3-month-old monkeys, whereas the density of PVBC boutons per pyramidal neuron did not differ between age groups. In contrast, relative levels of PV protein were approximately twofold higher in PVBC boutons in adult animals, whereas PV levels in PVChC boutons did not differ between age groups. These findings suggest cell type-specific mechanisms of maturation of PV-containing GABAergic boutons in monkey PFC.