CELL-GROWTH ON LIQUID INTERFACES - ROLE OF SURFACE-ACTIVE COMPOUNDS
CELL-GROWTH ON LIQUID INTERFACES - ROLE OF SURFACE-ACTIVE COMPOUNDS
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DOI：
10.1073/pnas.80.18.5622
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发表时间：
1983-01-01
期刊：
影响因子：
--
通讯作者：
GIAEVER, I
中科院分区：
文献类型：
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作者：
KEESE, CR;GIAEVER, I
锚定依赖性成纤维细胞可以通过使用在碳氟化合物流体和组织培养基之间的液-液界面处自发形成的蛋白质层作为基质来培养。为了使这种新型基质有效支撑汇合细胞层，蛋白质单层必须支撑成纤维细胞扩散所施加的应力。碳氟化合物流体的成分对蛋白质层的强度有显着影响，从而对细胞生长模式有显着影响。有证据表明，在纯化的碳氟化合物流体中不会形成足够坚固以支持细胞生长的蛋白质膜，但需要存在痕量的极性表面活性化合物。通过在经过氧化铝处理的氟碳流体中添加少量五氟苯甲酰氯，可以生产出优异的界面基材。这种理解被应用于生产能够普遍用于多种细胞（包括人成纤维细胞）的液体微载体系统。还描述了其中碳氟化合物被聚二甲基二苯基硅氧烷替代的微载体。
Anchorage-dependent fibroblasts can be cultured by using as a substrate the protein layer that spontaneously forms at the liquid-liquid interface between fluorocarbon fluids and tissue culture medium. For this novel substrate to be effective in supporting confluent cell layers, the protein monolayer must support the stresses exerted by spreading fibroblasts. The composition of the fluorocarbon fluid has a significant effect on the strength of the protein layer and, thus, on the patterns of cell growth. Evidence is presented demonstrating that a protein film, sufficiently strong to support cell growth, does not occur on purified fluorocarbon fluids but requires the presence of trace amounts of polar, surface active compounds. By the addition of small quantities of pentafluorobenzoyl chloride to alumina-treated fluorocarbon fluids, excellent interfacial substrates can be produced. This understanding was applied to produce a liquid microcarrier system capable of general use with a variety of cells, including human fibroblasts. A microcarrier in which the fluorocarbon is replaced with polydimethyldiphenyl siloxane is also described.