THE TOPOLOGY OF THE KINETOPLAST DNA NETWORK
THE TOPOLOGY OF THE KINETOPLAST DNA NETWORK
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DOI：
10.1016/0092-8674(95)90451-4
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发表时间：
1995-01-13
期刊：
影响因子：
64.5
通讯作者：
COZZARELLI, NR
中科院分区：
文献类型：
--
作者：
CHEN, JH;RAUCH, CA;COZZARELLI, NR
锥虫的动基体DNA（kDNA）是一个由5000个链状DNA微环和25个大环组成的网络。我们开发了以下策略来推导Crithidia fasciculata网络的微环的拓扑链接。首先，我们使用图论来提供可能的网络结构的精确模型。其次，在这些模型的基础上，我们预测了微环寡聚体的频率预期从随机网络断裂。第三，我们确定了kDNA网络的碎片模式作为消化程度的函数。第四，通过将结果与预测进行比较，我们确定了最能代表网络的模型。我们的结论是，每个微环平均连接到其他三个微环。结果可能是蜂窝状排列，每个微环通常位于六边形单元的顶点。这种拓扑结构对kDNA网络的组装、结构和功能都有影响。
Kinetoplast DNA (kDNA) of trypanosomatid parasites is a network of similar to 5000 catenated DNA minicircles and similar to 25 maxicircles. We developed the following strategy to deduce the topological linkage of the minicircles of the Crithidia fasciculata network. First, we used graph theory to provide precise models of possible network structures. Second, on the basis of these models, we predicted the frequencies of minicircle oligomers expected from random network breakage. Third, we determined the fragmentation pattern of kDNA networks as a function of the extent of digestion. Fourth, by comparison of the results with the predictions, we identified the model that best represents the network. We conclude that each minicircle is linked on average to three other minicircles. A honeycomb arrangement probably results, with each minicircle typically at the vertex of a hexagonal cell. This topology has implications for the assembly, structure, and function of kDNA networks.