Effect of toyF on wuyiencin and toyocamycin production by Streptomyces albulus CK-15
Effect of toyF on wuyiencin and toyocamycin production by Streptomyces albulus CK-15
复制标题
toyF对白色链霉菌CK15生产武夷菌素和丰加霉素的影响
DOI：
10.1007/s11274-022-03234-3
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发表时间：
2022-04-01
影响因子：
4.1
通讯作者：
Ge, Beibei
中科院分区：
文献类型：
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作者：
Liu, Binghua;Wei, Qiuhe;Ge, Beibei
白色链霉菌CK-15产生各种次级代谢产物，包括抗生素武夷菌素和丰霉素，据报道，它们可以控制广泛的植物真菌病害。CK-15中这些核苷类抗生素的产生受两个生物合成基因簇的调控。为了研究丰卡霉素生物合成对武夷菌素生产的潜在影响，我们在此构建了一株S.其中丰卡霉素生物合成基因簇中的关键基因即toyF缺失或过表达的白念珠菌菌株。toyF缺失突变体增加toyF不产生丰卡霉素，而武夷菌素的产量比野生型（WT）菌株提高了23.06%。此外，Delta toyF比WT菌株快4 h达到武夷菌素的最高产量水平（60 h vs.和64 h）。此外，toyF过表达菌株的丰卡霉素产量是WT菌株的两倍，而武夷菌素产量降低了29.10%。qRT-PCR显示，与WT株相比，丰霉素生物合成基因簇中的大多数基因在toyF增量中的表达水平较低，而武夷菌素生物合成基因簇中的基因表达水平上调。最后，在固体和液体培养基上培养时，toyF的生长速度远快于WT菌株。根据我们的研究结果，我们报告说，在工业发酵过程中，增量toyF有可能增加武夷菌素的产量，并减少发酵时间。
Streptomyces albulus CK-15 produces various secondary metabolites, including the antibiotics wuyiencin and toyocamycin, which can reportedly control a broad range of plant fungal diseases. The production of these nucleoside antibiotics in CK-15 is regulated by two biosynthesis gene clusters. To investigate the potential effect of toyocamycin biosynthesis on wuyiencin production, we herein generated S. albulus strains in which a key gene in the toyocamycin biosynthesis gene cluster, namely toyF, was either deleted or overexpressed. The toyF deletion mutant increment toyF did not produce toyocamycin, while the production of wuyiencin increased by 23.06% in comparison with that in the wild-type (WT) strain. In addition, Delta toyF reached the highest production level of wuyiencin 4 h faster than the WT strain (60 h vs. and 64 h). Further, toyocamycin production by the toyF overexpression strain was two-fold higher than by the WT strain, while wuyiencin production was reduced by 29.10%. qRT-PCR showed that most genes in the toyocamycin biosynthesis gene cluster were expressed at lower levels in increment toyF as compared with those in the WT strain, while the expression levels of genes in the wuyiencin biosynthesis gene cluster were upregulated. Finally, the growth rate of increment toyF was much faster than that of the WT strain when cultured on solid or liquid medium. Based on our findings, we report that in industrial fermentation processes, increment toyF has the potential to increase the production of wuyiencin and reduce the timeframe of fermentation.