In vitro effects of thyroxine and insulin on myoblasts from chick embryo skeletal muscle.
In vitro effects of thyroxine and insulin on myoblasts from chick embryo skeletal muscle.
复制标题
甲状腺素和胰岛素对鸡胚骨骼肌成肌细胞的体外影响。
DOI：
10.1016/0012-1606(80)90134-7
复制
发表时间：
1980
影响因子：
2.7
通讯作者：
T. Takuma
中科院分区：
文献类型：
--
作者：
M. Kumegawa;E. Ikeda;S. Hosoda;T. Takuma
本实验观察了胰岛素和L-甲状腺素（L-T4）对体外培养成肌细胞增殖和分化的影响。将超生理浓度的胰岛素或生理浓度的L-T_4加入到11日龄鸡胚大腿肌成肌细胞的无血清DM-153培养液中。虽然胰岛素的加入导致细胞总数的增加，融合的程度，和肌酸磷酸激酶（CPK）的活性，肌管变成球状结构，往往迅速退化。另一方面，虽然L-T4的加入对肌发生的影响较小，但肌管保持其分化状态的时间更长。此外，两种激素表现出协同效应。初始细胞密度的增加导致蛋白质和CPK活性的量增加，而不管激素的存在与否。提示胰岛素和L-T4对成肌作用不是分化特异性作用，而是细胞增殖的间接结果。
The role of insulin andl-thyroxine (L-T4) in stimulating myoblast proliferation and differentiation was investigatedin vitro. A superphysiological concentration of insulin or a physiological concentration of L-T4was added to cultures of myoblasts from 11-day-old chick embryo thigh muscle, grown in serum-free DM-153 medium. While the addition of insulin resulted in an increase in the total number of cells, in the extent of fusion, and in the creatine phosphokinase (CPK) activity, myotubes changed into globular structures which tended to degenerate rapidly. On the other hand, while the addition of L-T4had less effect on myogenesis, myotubes retained their differentiated state longer. Furthermore, the two hormones exhibited synergistic effects. An increase in the initial cell density resulted in an increase in the amount of protein and CPK activity, irrespective of the presence or absence of the hormones. This suggests that the effect of insulin and L-T4on myogenesis is not a differentiation-specific effect, but rather an indirect result of cell proliferation.