SPE-44 implements sperm cell fate.
SPE-44 implements sperm cell fate.
复制标题
DOI：
10.1371/journal.pgen.1002678
复制
发表时间：
2012
期刊：
影响因子：
4.5
通讯作者：
Smith HE
中科院分区：
文献类型：
--
作者：
Kulkarni M;Shakes DC;Guevel K;Smith HE
秀丽隐杆线虫两性生殖系的精子/卵母细胞决定为描述干细胞的命运特征和分化提供了有力的模型。控制配子命运的生殖系性别决定程序已经被很好地研究了，但对特定细胞类型转录的直接中介在很大程度上是未知的。我们发现Spe-44是精子基因表达的关键调节因子。SPE-44的缺失会导致精子在胞质分裂、细胞周期进展和细胞器组装方面的特异性缺陷，从而导致不育。Spe-44的表达与精子发生密切相关，并受生殖系性别决定途径的调控。SPE-44是数百个精子浓缩基因适当表达所必需的。SPE-44蛋白仅限于产生精子的生殖系，在那里它定位于常染色体(包含精子基因)，但被从转录沉默的X染色体(不包括)中排除。其他线虫物种中的同源基因也以性别偏见的方式表达，该蛋白在发育中的精子中同样表现出常染色体特异性的定位，强烈暗示在精子基因表达中具有进化上的保守作用。我们的分析首次发现了一个转录调节因子，它的主要功能是控制该系统中配子类型特定的转录。干细胞在生物体内产生了各种特殊的细胞类型。决定采用特定的细胞命运，这一过程被称为指定或确定，需要协调表达该特定细胞发育和正常运作所需的所有基因。了解支配这些基因表达模式的机制对于我们理解干细胞命运规范至关重要。我们在一种线虫物种中研究了这一过程，这种线虫物种从同一干细胞群体中同时产生精子和卵子。我们已经确定了一种名为spe-44的基因，它是精子基因(但不是卵子基因)正常表达所必需的。Spe-44的突变会产生带有发育缺陷的不育精子。SPE-44受控制精子/卵子决定的因素控制，其控制精子基因表达的功能在其他线虫物种中似乎是保守的。
The sperm/oocyte decision in the hermaphrodite germline of Caenorhabditis elegans provides a powerful model for the characterization of stem cell fate specification and differentiation. The germline sex determination program that governs gamete fate has been well studied, but direct mediators of cell-type-specific transcription are largely unknown. We report the identification of spe-44 as a critical regulator of sperm gene expression. Deletion of spe-44 causes sperm-specific defects in cytokinesis, cell cycle progression, and organelle assembly resulting in sterility. Expression of spe-44 correlates precisely with spermatogenesis and is regulated by the germline sex determination pathway. spe-44 is required for the appropriate expression of several hundred sperm-enriched genes. The SPE-44 protein is restricted to the sperm-producing germline, where it localizes to the autosomes (which contain sperm genes) but is excluded from the transcriptionally silent X chromosome (which does not). The orthologous gene in other Caenorhabditis species is similarly expressed in a sex-biased manner, and the protein likewise exhibits autosome-specific localization in developing sperm, strongly suggestive of an evolutionarily conserved role in sperm gene expression. Our analysis represents the first identification of a transcriptional regulator whose primary function is the control of gamete-type-specific transcription in this system. Stem cells give rise to the variety of specialized cell types within an organism. The decision to adopt a particular cell fate, a process known as specification or determination, requires the coordinated expression of all of the genes needed for that specialized cell to develop and function properly. Understanding the mechanisms that govern these patterns of gene expression is critical to our understanding of stem cell fate specification. We study this process in a nematode species that makes both sperm and eggs from the same stem cell population. We have identified a gene, named spe-44, that is required for the proper expression of sperm genes (but not egg genes). Mutation in spe-44 produces sterile sperm with developmental defects. spe-44 is controlled by factors that govern the sperm/egg decision, and its function in controlling sperm gene expression appears to be conserved in other nematode species.
登录
查看更多内容
影响因子：
64.5
作者：
GYURIS, J;GOLEMIS, E;BRENT, R
通讯作者：
BRENT, R
DOI：
10.1073/pnas.80.10.2926
发表时间：
1983-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
DAVIS, FM;TSAO, TY;RAO, PN
通讯作者：
RAO, PN
影响因子：
10.5
作者：
HODGKIN, J
通讯作者：
HODGKIN, J
DOI：
10.1038/89675
发表时间：
2001-07-01
期刊：
NATURE STRUCTURAL BIOLOGY
影响因子：
--
作者：
Bottomley, MJ;Collard, MW;Sattler, M
通讯作者：
Sattler, M
影响因子：
--
作者：
Kaul, S;Blackford, JA;Simons, SS
通讯作者：
Simons, SS