Cofilin/ADF is required for cell motility during Drosophila ovary development and oogenesis
Cofilin/ADF is required for cell motility during Drosophila ovary development and oogenesis
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DOI：
10.1038/35055120
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发表时间：
2001-02-01
影响因子：
21.3
通讯作者：
Laski, FA
中科院分区：
文献类型：
--
作者：
Chen, J;Godt, D;Laski, FA
细胞运动背后的驱动力是肌动蛋白细胞骨架。丝状伪足和板状伪足是由肌动蛋白丝向细胞膜聚合和延伸形成的（1，2）。在细丝的倒刺端的聚合通过在尖端的解聚来平衡，在“研磨-研磨”过程中回收肌动蛋白（2，3）。参与这一过程的一种蛋白质是cofilin/肌动蛋白解聚因子（ADF），它可以使肌动蛋白丝解聚，从而使肌动蛋白以更快的速度发生解聚（3，4）。Cofilin/ADF是肌动蛋白丝分解、胞质分裂和肌肉肌动蛋白丝组织所需的肌动蛋白结合蛋白（4-7）。也有证据表明cofilin/ADF增强细胞运动性（3，8，9），尽管尚未显示体内的直接需要。在这里，我们表明，果蝇cofilin/ADF（6，10），这是由双星（tsr）基因编码，促进卵巢发育和卵子发生过程中的细胞运动。在幼虫发育过程中，cofilin/ADF是形成终丝所需的细胞重排所必需的，终丝是对卵巢管起始很重要的体细胞堆叠。这些结果表明，cofilin/ADF是果蝇发育过程中肌动蛋白为基础的细胞运动的重要调节因子。
The driving force behind cell motility is the actin cytoskeleton. Filopodia and lamellipodia are formed by the polymerization and extension of actin filaments towards the cell membrane(1,2). This polymerization at the barbed end of the filament is balanced by depolymerization at the pointed end, recycling the actin in a 'tread-milling' process(2,3). One protein involved in this process is cofilin/actin-depolymerizing factor (ADF), which can depolymerize actin filaments, allowing treadmilling to occur at an accelerated rate(3,4). Cofilin/ADF is an actin-binding protein that is required for actin-filament disassembly, cytokinesis and the organization of muscle actin filaments(4-7). There is also evidence that cofilin/ADF enhances cell motility(3,8,9), although a direct requirement in vivo has not yet been shown. Here we show that Drosophila cofilin/ADF(6,10), which is encoded by the twin-star (tsr) gene, promotes cell movements during ovary development and oogenesis. During larval development, cofilin/ADF is required for the cell rearrangement needed for formation of terminal filaments, stacks of somatic cells that are important for the initiation of ovarioles. It is also required for the migration of border cells during oogenesis, These results show that cofilin/ADF is an important regulator of actin-based cell motility during Drosophila development.