DNA probe detection of periodontal pathogens.
DNA probe detection of periodontal pathogens.
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DNA探针检测牙周病原菌。
DOI：
10.1111/j.1399-302x.1986.tb00320.x
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发表时间：
1986
影响因子：
--
通讯作者：
K. K. Vaccaro
中科院分区：
文献类型：
--
作者：
C. French;E. Savitt;S. L. Simon;S. M. Eklund;M. C. Chen;L. Klotz;K. K. Vaccaro
使用同位素标记的放线共生放线杆菌、中间拟杆菌和牙龈拟杆菌的全基因组或物种特异性克隆脱氧核糖核酸（DNA）探针的核酸杂交技术可用于检测这些牙周病原体的少至102个细胞。该试验的灵敏度和特异性不受构建的混合培养物样品中存在5 × 108无关细菌的影响。目前使用非同位素试剂的可行性测试也可以在纯培养或混合培养中可靠和快速地检测这些口腔病原体。DNA探针应证明是有用的，在这些牙周病原体的快速，特异性和灵敏度的测定。
The technology for hybridization of nucleic acids using isotopically labeled whole genomic or species-specific cloned deoxyribonucleic acid (DNA) probes for Actinobacillus actinomycetemcomitans, Bacteroides intermedius and Bacleroides gingivulis can be used to detect as few as 102 cells of these periodontal pathogens. The sensitivity and specificity of the test is not affected by the presence of 5x 10s unrelated bacteria in constructed mixed culture samples. Current feasibility tests with non-isotopic reagents also give reliable and rapid detection of these oral pathogens in pure or mixed cultures. DNA probes should prove useful in a rapid, specific, and sensitive assay for these periodontal pathogens.