Wheat D-type cyclin Triae;CYCD2;1 regulate development of transgenic Arabidopsis plants
Wheat D-type cyclin Triae;CYCD2;1 regulate development of transgenic Arabidopsis plants
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小麦 D 型细胞周期蛋白 Triae;CYCD2;1 调节转基因拟南芥植物的发育
DOI：
10.1007/s00425-006-0294-0
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发表时间：
2006-10-01
期刊：
影响因子：
4.3
通讯作者：
Zhang, Xian Sheng
中科院分区：
文献类型：
--
作者：
Wang, Fang;Huo, Sheng Nan;Zhang, Xian Sheng
d型细胞周期蛋白基因在拟南芥植株发育中起关键作用。为了研究细胞周期蛋白在单子房中的功能，从小麦茎部组织中分离出一个推测的细胞周期蛋白D2基因，命名为Triae；CYCD2;1。Triae；CYCD2转录本；1主要分布在小麦植株的增殖组织中，尤其是茎尖分生组织和叶片原基。Triae；CYCD2异位表达；1在拟南芥中影响植株形态，延缓植株生长。进一步的研究表明，在转基因植物和组织中都发生了促进细胞分裂和抑制细胞分化的作用。体外实验表明，Triae;CYCD2；1对细胞分裂素和生长素有应答作用。分子分析显示，几种细胞周期相关基因的转录水平，特别是Arath， CYCD3；1、在拟南芥植株中表达Triae；CYCD2；本研究结果为小麦d型细胞周期蛋白的研究提供了新的信息。
The D-type cyclin genes play key roles in plant development of Arabidopsis. To investigate the functions of cyclins in monocots, a putative ortholog of cyclin D2 genes was isolated from wheat shoot tissues, and designated as Triae;CYCD2;1. The transcripts of Triae;CYCD2;1 were primarily localized in the proliferating tissues, particularly, in shoot apical meristem and leaf primordia of wheat plants. Ectopic expression of Triae;CYCD2;1 in Arabidopsis affected plant morphology and retarded plant growth. Further examination showed that the promotion of cell division and the inhibition of cell differentiation occurred in both transgenic plants and tissues. In vitro experiments indicated that Triae;CYCD2;1 had functional roles in responding to cytokinin and auxin. Molecular analysis revealed that the transcript levels of several cell cycle-associated genes, particularly Arath;CYCD3;1, were increased in the Arabidopsis plants with the expressing Triae;CYCD2;1. The results in this study provide new information on D-type cyclin in wheat.