Somatic embryogenesis and plant regeneration in centipedegrass (Eremochloa ophiuroides [Munro] Hack.)
Somatic embryogenesis and plant regeneration in centipedegrass (Eremochloa ophiuroides [Munro] Hack.)
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DOI：
10.1007/s11627-008-9115-4
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发表时间：
2008-04-01
影响因子：
2.6
通讯作者：
Wu, Hong
中科院分区：
文献类型：
--
作者：
Liu, Mingxi;Yang, Jing;Wu, Hong
假俭草是重要的暖季型草坪草和牧草。为探索生物技术在假俭草育种中的应用潜力，建立了假俭草高效植株再生体系。研究了4种基本培养基和2,4种2，4-D和6-苄基腺嘌呤（BAP）组合对成熟种子外植体愈伤组织诱导的影响。本文研究了20种萘乙酸（NAA）和BAP组合对再生植株的影响。结果表明，在Murashige和Skoog基本培养基中添加4.5mg l（-1）2，4-D和1 mg l（-1）BAP是诱导愈伤组织的最佳培养基，而2 mg l（-1）BAP和1 mg l（-1）NAA的组合诱导的芽和根的再生率最高。本研究为假俭草的体细胞无性系变异和遗传转化育种奠定了基础。
Centipedegrass (Eremochloa ophiuroides [Munro] Hack.) is an important warm-season turfgrass and pasture grass. To explore the potential use of biotechnical tools in breeding of centipedegrass, we established an efficient plant regeneration system for this species. Four basal media and 24 combinations of 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzyladenine (BAP) were examined for their effects on callus induction from mature seed explants. Twenty combinations of naphthaleneacetic acid (NAA) and BAP were tested for their effect on plant regeneration. Results indicated that Murashige and Skoog basal medium supplemented with 4.5 mg l(-1) 2,4-D and 1 mg l(-1) BAP was the best medium for callus induction, while the combination of 2 mg l(-1) BAP and 1 mg l(-1) NAA induced the highest rate of regeneration and development of shoots and roots. This work provides a basis for the breeding of centipedegrass through somaclonal variation and genetic transformation.