Plant regeneration from hairy roots induced by infection with Agrobacterium rhizogenes in Crotalaria juncea L.
Plant regeneration from hairy roots induced by infection with Agrobacterium rhizogenes in Crotalaria juncea L.
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DOI：
10.1007/s002990050774
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发表时间：
2000-05
影响因子：
6.2
通讯作者：
A. Ohara;Yoko Akasaka;Hiroyuki Daimon;M. Mii
中科院分区：
文献类型：
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作者：
A. Ohara;Yoko Akasaka;Hiroyuki Daimon;M. Mii
以抗线虫的绿色肥料作物野百合（Crotalariajuncea）叶片为材料，用发根农杆菌（AgrobacteriumrhizogenesA 13）（MAFF 02 -10266）的一株米西莫平型野生菌侵染，诱导其产生毛状根。这些根表现出旺盛的生长和丰富的侧枝半强度Murashige和Skoog（1/2 MS）培养基上没有植物激素。将30%的根段转移到含有3 mg/l苄基腺嘌呤的培养基上3个月后，诱导出不定芽。这些芽转移到不含植物激素的1.2%琼脂固化的1/2 MS培养基上1个月后生根。再生植物在温室条件下成功生长。Southern印迹分析证实了再生植株的转基因性质。
Hairy roots were induced from leaf segments ofCrotalaria juncea,which is used as a green manure crop antagonistic to nematodes, by infection with a mikimopine type wild strain ofAgrobacterium rhizogenesA13 (MAFF02-10266). These roots exhibited vigorous growth and abundant lateral branching on half-strength Murashige and Skoog (1/2MS) medium without phytohormones. The adventitious shoots were induced from 30% of root segments 3 months after transfer onto medium containing 3 mg/l benzyl adenine. These shoots produced roots 1 month after transfer onto 1.2% agar-solidified 1/2MS medium without phytohormones. Regenerated plants were successfully grown under greenhouse conditions. The transgenic nature of the regenerated plants was confirmed by Southern-blot analysis.