A Field-Deployable and Low-Cost PCR (FLC-PCR) Thermocycler for the Rapid Detection of Environmental E. coli
A Field-Deployable and Low-Cost PCR (FLC-PCR) Thermocycler for the Rapid Detection of Environmental E. coli
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用于快速检测环境大肠杆菌的可现场部署的低成本 PCR (FLC-PCR) 热循环仪
DOI：
10.1109/embc44109.2020.9176519
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Li, Yiyan
中科院分区：
文献类型：
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作者：
Ferguson, James;Duran, Jesse;Killinen, Wesley;Wagner, Jason;Kulesza, Caroline;Chatterley, Christie;Li, Yiyan
本研究旨在开发一种可现场部署的、低成本的PCR热循环仪（FLC-PCR），以进行聚合酶链反应（PCR）快速检测环境中的大肠杆菌。杆菌四个高效（77.1 W）的珀尔帖模块被用作中央温度控制单元。一个250 W硅胶加热垫用于加热盖。PID（比例积分微分）控制算法的热循环是由一个低成本的8位，16 MHz的微控制器（ATMEGA 328 P-PU）。ybbW和uidA基因来自特异的E.大肠杆菌菌落用作PCR反应的扩增子，所述PCR反应通过商业PCR机器（Bio-Rad）和我们的FLC-PCR热循环仪进行。加热和冷却速度平均为1.11 ± 0.33°C/s，这与商业台式PCR热循环仪相当，并且加热盖的效率优于Bio-Rad PCR热循环仪。该系统的总成本低于200美元，比市售设备低十倍以上。加热块可以定制以适应不同的PCR管甚至微流体室。将使用8000 W便携式发电机作为现场研究的电源。
This is a proof-of-concept study for the development of a field-deployable and low-cost PCR thermocycler (FLC-PCR) to perform Polymerase Chain Reaction (PCR) for the rapid detection of environmental E. coli. Four efficient (77.1 W) peltier modules are used as the central temperature control unit. One 250 W silicone heating pad is used for the heating lid. The PID (proportional-integral-derivative) control algorithm for the thermocycles is implemented by a low-cost 8-bit, 16 MHz microcontroller (ATMEGA328P-PU). ybbW and uidA genes from specific E. coli colonies were used as amplicons for the PCR reactions that were carried out by a commercial PCR machine (Bio-Rad) and our FLC-PCR thermocycler. The heating and cooling speeds averaged 1.11 ± 0.33°C/s which is on a par with the commercial bench-top PCR thermocycler and the efficiency of the heating lid outperformed the Bio-Rad PCR thermocycler. The overall cost of the system is lower than $200 which is more than ten times lower than commercially available units. The heating block can be customized to accommodate different PCR tubes and even microfluidic chambers. An 8000 W portable power generator will be used as the power supply for field studies.